Overview

  • Product name

    Anti-UBQLN2 antibody [6H9]
    See all UBQLN2 primary antibodies
  • Description

    Mouse monoclonal [6H9] to UBQLN2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Human UBQLN2. Expressed in and purified from E.coli.
    Database link: Q9UHD9

  • Positive control

    • WB: NIH/3T3, C6, HEK-293, HeLa and SH-SY5Y cell lysates; Rat and mouse whole brain lysates; HeLa and 3T3 cell lysates; Transfected primary mouse neuron and glia cell cultures. ICC/IF: NIH/3T3 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab190283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/1000 - 1/2000. Predicted molecular weight: 66 kDa.
IHC-P 1/1000.

Target

  • Relevance

    UBQLN2 (Ubiquilin 2) is a ubiquitin-like protein which contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Ubiquilins interact with both proteasomes and ubiquitin ligases, and are thought to link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. UBQLN2 increases the half-life of proteins destined to be degraded by the proteasome.
  • Cellular localization

    Cytoplasmic and Nuclear. Note=Where it colocalizes with the proteasome. Associated with fibers in mitotic cells.
  • Database links

  • Alternative names

    • bA431N15.1 antibody
    • CHAP 1 antibody
    • CHAP1 antibody
    • CHAP1/DSK2 antibody
    • Dsk 2 antibody
    • Dsk2 antibody
    • DSK2 homolog antibody
    • hPLIC 2 antibody
    • hPLIC2 antibody
    • HRIHFB2157 antibody
    • LIC 2 antibody
    • LIC2 antibody
    • N4BP4 antibody
    • Nedd4 binding protein 4 antibody
    • PLIC 2 antibody
    • PLIC2 antibody
    • Protein linking IAP with cytoskeleton 2 antibody
    • RIHFB2157 antibody
    • Ubiquilin 2 antibody
    • Ubiquilin2 antibody
    • Ubiquitin like product Chap1/Dsk2 antibody
    • UBQLN 2 antibody
    see all

Images

  • NIH/3T3 (mouse embyro fibroblast cell line) cells stained for UBQLN2 (green) using ab190283 at 1/1000 dilution in ICC/IF. Costained with chicken pAb to lamin A/C at 1/5,000 dilution (red). The blue is DAPI staining of nuclear DNA. 

  • All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 2 : C6 (rat glial tumor cell line) cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 6 : Rat whole brain lysate
    Lane 7 : Mouse whole brain lysate

    Predicted band size: 66 kDa

  • All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution

    Lane 1 : Primary mouse neuron and glia cell cultures
    Lane 2 : Primary mouse neuron and glia cells transduced with Human ubiquilin 2 wild type
    Lane 3 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P506T mutant
    Lane 4 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P497S mutant
    Lane 5 : Primary mouse neuron and glia cell cultures transduced with enhanced GFP control
    Lane 6 : HeLa cell lysate
    Lane 7 : 3T3 cell lysate

    Predicted band size: 66 kDa

  • Immunofluorescent analysis of HeLa cells labeling UBQLN2 with ab190283 at 1/1000 dilution. Vimentin is stained in red.

References

This product has been referenced in:

  • Farrawell NE  et al. Distinct partitioning of ALS associated TDP-43, FUS and SOD1 mutants into cellular inclusions. Sci Rep 5:13416 (2015). Read more (PubMed: 26293199) »
See 1 Publication for this product

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