Anti-UBQLN2 antibody (ab217056)
Key features and details
- Rabbit polyclonal to UBQLN2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBQLN2 antibody
See all UBQLN2 primary antibodies -
Description
Rabbit polyclonal to UBQLN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human UBQLN2 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UHD9 -
Positive control
- HeLa, 293T and Jurkat cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab217056 was affinity purified using an epitope specific to UBQLN2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
UBQLN2 (Ubiquilin 2) is a ubiquitin-like protein which contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Ubiquilins interact with both proteasomes and ubiquitin ligases, and are thought to link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. UBQLN2 increases the half-life of proteins destined to be degraded by the proteasome. -
Cellular localization
Cytoplasmic and Nuclear. Note=Where it colocalizes with the proteasome. Associated with fibers in mitotic cells. -
Database links
- Entrez Gene: 29978 Human
- Entrez Gene: 54609 Mouse
- Omim: 300264 Human
- SwissProt: Q9UHD9 Human
- SwissProt: Q9QZM0 Mouse
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Alternative names
- bA431N15.1 antibody
- CHAP 1 antibody
- CHAP1 antibody
see all
Images
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Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling UBQLN2 using ab217056 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated UBQLN2, ab217056 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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All lanes : Anti-UBQLN2 antibody (ab217056) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutescells prepared using NETN lysis buffer
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab217056 has been referenced in 1 publication.
- Roboti P et al. Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex. J Cell Sci 135:N/A (2022). PubMed: 35543156