Overview

  • Product name

  • Description

    Rabbit polyclonal to UBQLN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human UBQLN2 aa 1-50. The exact sequence is proprietary.
    Sequence:

    MAENGESSGP PRPSRGPAAA QGSAAAPAEP KIIKVTVKTP KEKEEFAVPE


    Database link: Q9UHD9

  • Positive control

    • HeLa, 293T and Jurkat cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab217056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    UBQLN2 (Ubiquilin 2) is a ubiquitin-like protein which contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Ubiquilins interact with both proteasomes and ubiquitin ligases, and are thought to link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. UBQLN2 increases the half-life of proteins destined to be degraded by the proteasome.
  • Cellular localization

    Cytoplasmic and Nuclear. Note=Where it colocalizes with the proteasome. Associated with fibers in mitotic cells.
  • Database links

  • Alternative names

    • bA431N15.1 antibody
    • CHAP 1 antibody
    • CHAP1 antibody
    • CHAP1/DSK2 antibody
    • Dsk 2 antibody
    • Dsk2 antibody
    • DSK2 homolog antibody
    • hPLIC 2 antibody
    • hPLIC2 antibody
    • HRIHFB2157 antibody
    • LIC 2 antibody
    • LIC2 antibody
    • N4BP4 antibody
    • Nedd4 binding protein 4 antibody
    • PLIC 2 antibody
    • PLIC2 antibody
    • Protein linking IAP with cytoskeleton 2 antibody
    • RIHFB2157 antibody
    • Ubiquilin 2 antibody
    • Ubiquilin2 antibody
    • Ubiquitin like product Chap1/Dsk2 antibody
    • UBQLN 2 antibody
    see all

Images

  • Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling UBQLN2 using ab217056 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated UBQLN2, ab217056 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

  • All lanes : Anti-UBQLN2 antibody (ab217056) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 3 minutes


    cells prepared using NETN lysis buffer

References

ab217056 has not yet been referenced specifically in any publications.

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