Key features and details
- Rabbit polyclonal to UBQLN2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBQLN2 antibody
See all UBQLN2 primary antibodies
DescriptionRabbit polyclonal to UBQLN2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- HeLa, 293T and Jurkat cell lysates
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217056 was affinity purified using an epitope specific to UBQLN2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab217056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceUBQLN2 (Ubiquilin 2) is a ubiquitin-like protein which contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Ubiquilins interact with both proteasomes and ubiquitin ligases, and are thought to link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. UBQLN2 increases the half-life of proteins destined to be degraded by the proteasome.
Cellular localizationCytoplasmic and Nuclear. Note=Where it colocalizes with the proteasome. Associated with fibers in mitotic cells.
- bA431N15.1 antibody
- CHAP 1 antibody
- CHAP1 antibody
Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling UBQLN2 using ab217056 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated UBQLN2, ab217056 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-UBQLN2 antibody (ab217056) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes
cells prepared using NETN lysis buffer
ab217056 has not yet been referenced specifically in any publications.