Key features and details
- Rabbit polyclonal to UBQLN2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBQLN2 antibody
See all UBQLN2 primary antibodies
DescriptionRabbit polyclonal to UBQLN2
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human UBQLN2 aa 500-550. The exact sequence is proprietary.
Database link: Q9UHD9
- IP: UBQLN2 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245553 was affinity purified using an epitope specific to UBQLN2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245553 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceUBQLN2 (Ubiquilin 2) is a ubiquitin-like protein which contains an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Ubiquilins interact with both proteasomes and ubiquitin ligases, and are thought to link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. UBQLN2 increases the half-life of proteins destined to be degraded by the proteasome.
Cellular localizationCytoplasmic and Nuclear. Note=Where it colocalizes with the proteasome. Associated with fibers in mitotic cells.
- bA431N15.1 antibody
- CHAP 1 antibody
- CHAP1 antibody
UBQLN2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245553 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab245553 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 3 minutes.
ab245553 has not yet been referenced specifically in any publications.