Key features and details
- Rabbit polyclonal to UBR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBR1 antibody
See all UBR1 primary antibodies
DescriptionRabbit polyclonal to UBR1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between amino acids 1125-1175 of Human UBR1 (NP_777576.1).
- Jurkat whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab108215 was affinity purified using an epitope specific to UBR1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab108215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 200 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionE3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.
Tissue specificityBroadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis.
Sequence similaritiesBelongs to the UBR1 family.
Contains 1 RING-type zinc finger.
Contains 1 UBR-type zinc finger.
Developmental stageExpressed in fetal pancreas.
DomainThe RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- E3 ubiquitin-protein ligase UBR1 antibody
- JBS antibody
- N-recognin-1 antibody
All lanes : Anti-UBR1 antibody (ab108215) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 200 kDa
Exposure time: 3 minutes
Detection of UBR1 by Western Blot of Immunprecipitate.
Lane 1: ab108215 at 1µg/ml staining UBR1 in Jurkat whole cell lysate immunoprecipitated using ab108215 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
ab108215 has not yet been referenced specifically in any publications.