Anti-UBR1 antibody (ab138267)
Key features and details
- Rabbit polyclonal to UBR1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBR1 antibody
See all UBR1 primary antibodies -
Description
Rabbit polyclonal to UBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to an internal region of Human UBR1 (UniProt Q8IWV7).
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Positive control
- HUVEC cell lysate
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab138267 was affinity purified from rabbit antiserum by immunogenic peptide affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 200 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 200 kDa. |
Target
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Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis. -
Sequence similarities
Belongs to the UBR1 family.
Contains 1 RING-type zinc finger.
Contains 1 UBR-type zinc finger. -
Developmental stage
Expressed in fetal pancreas. -
Domain
The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 197131 Human
- Entrez Gene: 22222 Mouse
- Omim: 605981 Human
- SwissProt: Q8IWV7 Human
- SwissProt: O70481 Mouse
- Unigene: 591121 Human
- Unigene: 389330 Mouse
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Alternative names
- E3 ubiquitin-protein ligase UBR1 antibody
- JBS antibody
- N-recognin-1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab138267 has been referenced in 1 publication.
- Pore SK et al. N-end rule pathway inhibitor sensitizes cancer cells to antineoplastic agents by regulating XIAP and RAD21 protein expression. J Cell Biochem 121:804-815 (2020). PubMed: 31407360