• Product name

  • Description

    Rabbit polyclonal to UBR1
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human UBR1 aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab42419)

  • Positive control

    • Skeletal Muscle (Human) Tissue Lysate - adult normal tissue HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate HeLa (Human epithelial carcinoma cell line) Nuclear Lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab42420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).


  • Function

    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.
  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis.
  • Sequence similarities

    Belongs to the UBR1 family.
    Contains 1 RING-type zinc finger.
    Contains 1 UBR-type zinc finger.
  • Developmental stage

    Expressed in fetal pancreas.
  • Domain

    The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase UBR1 antibody
    • JBS antibody
    • N-recognin-1 antibody
    • ubiquitin protein ligase E3 component n-recognin 1 antibody
    • Ubiquitin-protein ligase E3-alpha-1 antibody
    • Ubiquitin-protein ligase E3-alpha-I antibody
    • UBR1 antibody
    • UBR1_HUMAN antibody
    see all


  • All lanes : Anti-UBR1 antibody (ab42420) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 200 kDa
    Observed band size: 200 kDa
    Additional bands at: 100 kDa (possible isoform), 100 kDa (possible post-translational modification), 93 kDa (possible isoform)


This product has been referenced in:

  • Leng S  et al. Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung. Cancer Res 75:3108-17 (2015). Read more (PubMed: 26183928) »
See 1 Publication for this product

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