Overview

  • Product name

  • Description

    Goat polyclonal to UBR2
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human UBR2 aa 1618-1631. NP_056070.1.
    Sequence:

    SCPKSGGDKSRAPT


    Database link: Q8IWV8

  • Positive control

    • Human brain, cortex tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 201 kDa.

Preliminary experiments gave bands at approx 150kDa and 100kDa in Human Lung lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 201kDa according to NP_056070.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Target

  • Relevance

    UBR2 (Ubiquitin protein ligase E3 component n-recognin 2) is an E3 ubiquitin-protein ligase involved in the N-end rule pathway. It recognizes and binds to proteins with destabilizing N-terminal residues (an N-terminal degron), leading to their ubiquitination and subsequent degradation.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • bA49A4.1 antibody
    • C6orf133 antibody
    • dJ242G1.1 antibody
    • dJ392M17.3 antibody
    • DKFZp686C08114 antibody
    • E3 ubiquitin protein ligase UBR2 antibody
    • KIAA0349 antibody
    • MGC71112 antibody
    • N recognin 2 antibody
    • OTTHUMP00000016403 antibody
    • OTTHUMP00000039767 antibody
    • OTTHUMP00000039768 antibody
    • RP3 392M17.3 antibody
    • Ubiquitin ligase E3 alpha II antibody
    • Ubiquitin protein ligase E3 alpha 2 antibody
    • Ubiquitin protein ligase E3 alpha II antibody
    • Ubiquitin protein ligase E3 component n recognin 2 antibody
    • UBR 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling UBR2 with ab211275 at 5 µg/ml.

References

ab211275 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211275.
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