Key features and details
- Rabbit polyclonal to UBR2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UBR2 antibody
See all UBR2 primary antibodies
DescriptionRabbit polyclonal to UBR2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityProtein A purified
Purification notesab217069 was affinity purified using an epitope specific to UBR2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab217069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 201 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceUBR2 (Ubiquitin protein ligase E3 component n-recognin 2) is an E3 ubiquitin-protein ligase involved in the N-end rule pathway. It recognizes and binds to proteins with destabilizing N-terminal residues (an N-terminal degron), leading to their ubiquitination and subsequent degradation.
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All lanes : Anti-UBR2 antibody (ab217069) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 201 kDa
Exposure time: 75 seconds
Detection of UBR2 in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded). ab217069 was used at 6 µg/mg lysate for IP (Lane 1), and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: chemiluminescence with an exposure time of 30 seconds.
ab217069 has not yet been referenced specifically in any publications.