Overview

  • Product name

  • Description

    Rabbit polyclonal to UBR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UBR2 aa 1705-1755. The exact sequence is proprietary.
    Sequence:

    DQGLRR GNPLHLCKER FKKIQKLWHQ HSVTEEIGHA QEANQTLVGI DWQHL


    Database link: Q8IWV8

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab217069 was affinity purified using an epitope specific to UBR2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 201 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    UBR2 (Ubiquitin protein ligase E3 component n-recognin 2) is an E3 ubiquitin-protein ligase involved in the N-end rule pathway. It recognizes and binds to proteins with destabilizing N-terminal residues (an N-terminal degron), leading to their ubiquitination and subsequent degradation.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • bA49A4.1 antibody
    • C6orf133 antibody
    • dJ242G1.1 antibody
    • dJ392M17.3 antibody
    • DKFZp686C08114 antibody
    • E3 ubiquitin protein ligase UBR2 antibody
    • KIAA0349 antibody
    • MGC71112 antibody
    • N recognin 2 antibody
    • OTTHUMP00000016403 antibody
    • OTTHUMP00000039767 antibody
    • OTTHUMP00000039768 antibody
    • RP3 392M17.3 antibody
    • Ubiquitin ligase E3 alpha II antibody
    • Ubiquitin protein ligase E3 alpha 2 antibody
    • Ubiquitin protein ligase E3 alpha II antibody
    • Ubiquitin protein ligase E3 component n recognin 2 antibody
    • UBR 2 antibody
    see all

Images

  • All lanes : Anti-UBR2 antibody (ab217069) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : mouse TCMK1 whole cell lysate
    Lane 5 : mouse NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 201 kDa


    Exposure time: 75 seconds
  • Detection of UBR2 in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded). ab217069 was used at 6 µg/mg lysate for IP (Lane 1), and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: chemiluminescence with an exposure time of 30 seconds.

References

ab217069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab217069.
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