Anti-UBR4/p600 antibody (ab86738)
Key features and details
- Rabbit polyclonal to UBR4/p600
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBR4/p600 antibody
See all UBR4/p600 primary antibodies -
Description
Rabbit polyclonal to UBR4/p600 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to Human UBR4/p600 aa 5133-5183.
Database link: NP_065816.1 -
Positive control
- WB: HeLa whole cell lysate (ab150035) IHC-P: human prostate carcinoma tissue
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General notes
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 574 kDa.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 574 kDa. |
Target
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Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the UBR4 family.
Contains 1 UBR-type zinc finger. -
Cellular localization
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility. - Information by UniProt
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Database links
- Entrez Gene: 23352 Human
- Entrez Gene: 69116 Mouse
- Entrez Gene: 313658 Rat
- Omim: 609890 Human
- SwissProt: Q5T4S7 Human
- SwissProt: A2AN08 Mouse
- SwissProt: Q2TL32 Rat
- Unigene: 148078 Human
see all -
Alternative names
- 600 kDa retinoblastoma protein associated factor antibody
- 600 kDa retinoblastoma protein-associated factor antibody
- E3 ubiquitin-protein ligase UBR4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4/p600 with ab86738 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-UBR4/p600 antibody (ab86738) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 574 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab86738 has been referenced in 12 publications.
- Hunt LC et al. Antagonistic control of myofiber size and muscle protein quality control by the ubiquitin ligase UBR4 during aging. Nat Commun 12:1418 (2021). PubMed: 33658508
- Kaiho-Soma A et al. TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. Mol Cell 81:1411-1424.e7 (2021). PubMed: 33567268
- Tian M et al. ACLY ubiquitination by CUL3-KLHL25 induces the reprogramming of fatty acid metabolism to facilitate iTreg differentiation. Elife 10:N/A (2021). PubMed: 34491895
- Leboeuf D et al. The Arg/N-Degron Pathway-A Potential Running Back in Fine-Tuning the Inflammatory Response? Biomolecules 10:N/A (2020). PubMed: 32545869
- Hunt LC et al. A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice. Cell Rep 28:1268-1281.e6 (2019). PubMed: 31365869