Overview

  • Product name

  • Description

    Rabbit polyclonal to UBR4/p600
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to Human UBR4/p600 aa 5133-5183.
    Database link: NP_065816.1

  • Positive control

  • General notes

    Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

    Previously labelled as UBR4. 

Properties

Applications

Our Abpromise guarantee covers the use of ab86738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 574 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the UBR4 family.
      Contains 1 UBR-type zinc finger.
    • Cellular localization

      Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility.
    • Information by UniProt
    • Database links

    • Alternative names

      • 600 kDa retinoblastoma protein associated factor antibody
      • 600 kDa retinoblastoma protein-associated factor antibody
      • E3 ubiquitin-protein ligase UBR4 antibody
      • KIAA0462 antibody
      • KIAA1307 antibody
      • N-recognin-4 antibody
      • p600 antibody
      • RBAF600 antibody
      • Retinoblastoma associated factor 600 like protein antibody
      • Retinoblastoma associated factor of 600 kDa antibody
      • Retinoblastoma-associated factor 600 antibody
      • Retinoblastoma-associated factor of 600 kDa antibody
      • Ubiquitin protein ligase E3 component n recognin 4 antibody
      • UBR4 antibody
      • UBR4_HUMAN antibody
      • Zinc finger UBR1 type protein 1 antibody
      • Zinc finger UBR1-type protein 1 antibody
      • Zinc finger, UBR1 type 1 antibody
      • ZUBR 1 antibody
      • ZUBR1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4/p600 with ab86738 at 1/200 (1µg/ml). Detection: DAB.

    • All lanes : Anti-UBR4/p600 antibody (ab86738) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 574 kDa


      Exposure time: 30 seconds

    References

    This product has been referenced in:

    • Kim ST  et al. The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome. J Cell Sci 131:N/A (2018). Read more (PubMed: 30111582) »
    • Kim ST  et al. The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins. PLoS One 13:e0202260 (2018). Read more (PubMed: 30157281) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

    Dr. James Dachtler

    Verified customer

    Submitted Jul 22 2015

    Application
    Western blot
    Sample
    Drosophila melanogaster Cell lysate - whole cell (Whole abdomen)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Whole abdomen
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Paromita Gupta

    Verified customer

    Submitted Nov 25 2014

    Answer

    Thank you for contacting us regarding ab86738. I am sorry that you have been experiencing difficulties with this antibody in WB. Attached please find a complete protocol as well as the gel type information. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

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