Key features and details
- Rabbit polyclonal to UBR7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBR7 antibody
See all UBR7 primary antibodies
DescriptionRabbit polyclonal to UBR7
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human UBR7 (internal sequence). The exact sequence is proprietary.
Database link: Q8N806
- ICC/IF: MCF7 cells. IHC-P: Huh-7 hepatocellular carcinoma xenograft tissue. WB: U-87 MG whole cell lysate.
Previously labelled as C14orf130.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceUBR7 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
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MCF7 (human breast adenocarcinoma cell line) cells stained for UBR7 (green) using ab228911 at 1/200 dilution in ICC/IF. Cells were fixed in paraformaldehyde.
Blue: Hoechst 33342 staining.
Paraffin-embedded Huh-7 hepatocellular carcinoma xenograft stained for UBR7 using ab228911 at 1/500 dilution in immunohistochemical analysis.
Anti-UBR7 antibody (ab228911) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Predicted band size: 48 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228911 has not yet been referenced specifically in any publications.