Overview

  • Product name

  • Description

    Rabbit polyclonal to UBR7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human UBR7 (internal sequence). The exact sequence is proprietary.
    Database link: Q8N806

  • Positive control

    • ICC/IF: MCF7 cells. IHC-P: Huh-7 hepatocellular carcinoma xenograft tissue. WB: U-87 MG whole cell lysate.
  • General notes

    Previously labelled as C14orf130.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    UBR7 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
  • Database links

  • Alternative names

    • 5730410I19Rik antibody
    • AA589405 antibody
    • AW557761 antibody
    • C14orf130 antibody
    • Chromosome 14 open reading frame 130 antibody
    • EC 6.3.2.- antibody
    • FLJ10483 antibody
    • MGC94098 antibody
    • MGC9518 antibody
    • N recognin 7 antibody
    • Putative E3 ubiquitin-protein ligase UBR7 antibody
    • RGD1359144 antibody
    • Ubiquitin protein ligase E3 component n-recognin 7 (putative) antibody
    • UBR7 antibody
    see all

Images

  • MCF7 (human breast adenocarcinoma cell line) cells stained for UBR7 (green) using ab228911 at 1/200 dilution in ICC/IF. Cells were fixed in paraformaldehyde.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded Huh-7 hepatocellular carcinoma xenograft stained for UBR7 using ab228911 at 1/500 dilution in immunohistochemical analysis.

  • Anti-UBR7 antibody (ab228911) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    12% SDS-PAGE gel.

References

ab228911 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228911.
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