Overview

  • Product name

  • Description

    Rabbit polyclonal to UBR7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human UBR7 aa 1-50. The exact sequence is proprietary. NP_786924.2
    Database link: Q8N806

  • Positive control

    • WB: HEK-293T, HeLa and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000.

Target

  • Relevance

    UBR7 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
  • Database links

  • Alternative names

    • 5730410I19Rik antibody
    • AA589405 antibody
    • AW557761 antibody
    • C14orf130 antibody
    • Chromosome 14 open reading frame 130 antibody
    • EC 6.3.2.- antibody
    • FLJ10483 antibody
    • MGC94098 antibody
    • MGC9518 antibody
    • N recognin 7 antibody
    • Putative E3 ubiquitin-protein ligase UBR7 antibody
    • RGD1359144 antibody
    • Ubiquitin protein ligase E3 component n-recognin 7 (putative) antibody
    • UBR7 antibody
    see all

Images

  • UBR7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab241371 at 6µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241371 at 0.1 µg/ml.

    Lane 1: ab241371 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

     

  • All lanes : Anti-UBR7 antibody (ab241371) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 30 seconds

References

ab241371 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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