Anti-UBXD8 antibody (ab154064)
Overview
-
Product name
Anti-UBXD8 antibody
See all UBXD8 primary antibodies -
Description
Rabbit polyclonal to UBXD8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Recombinant fragment corresponding to Human UBXD8 aa 176-393.
-
Positive control
- 293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue
-
General notes
This product was previously labelled as ETEA
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. -
Tissue specificity
Broadly expressed, with highest levels in brain. -
Sequence similarities
Contains 1 UBA domain.
Contains 1 UBX domain. -
Cellular localization
Cytoplasm. Lipid droplet. Endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 767948 Cow
- Entrez Gene: 23197 Human
- Entrez Gene: 76577 Mouse
- Entrez Gene: 291000 Rat
- SwissProt: Q96CS3 Human
- SwissProt: Q3TDN2 Mouse
- SwissProt: Q5BK32 Rat
- Unigene: 484242 Human
see all -
Alternative names
- AI462440 antibody
- expressed in T cells and eosinophils in atopic dermatitis antibody
- faf2 antibody
see all
Images
-
Western blot - Anti-UBXD8 antibody (ab154064)Anti-UBXD8 antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.
Protocols
References
ab154064 has been referenced in 1 publication.
- Wang T et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237
