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    ubxd8-antibody-ab154064.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Anti-UBXD8 antibody (ab154064)

  • Datasheet
  • SDS
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Western blot - Anti-UBXD8 antibody (ab154064)
  • Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)

Key features and details

  • Rabbit polyclonal to UBXD8
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-UBXD8 antibody
    See all UBXD8 primary antibodies
  • Description

    Rabbit polyclonal to UBXD8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human UBXD8 aa 176-393.

  • Positive control

    • 293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue
  • General notes

     This product was previously labelled as ETEA

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab30128)
    • A-431 whole cell lysate (ab30132)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • Tissue specificity

    Broadly expressed, with highest levels in brain.
  • Sequence similarities

    Contains 1 UBA domain.
    Contains 1 UBX domain.
  • Cellular localization

    Cytoplasm. Lipid droplet. Endoplasmic reticulum.
  • Target information above from: UniProt accession Q96CS3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 767948 Cow
    • Entrez Gene: 23197 Human
    • Entrez Gene: 76577 Mouse
    • Entrez Gene: 291000 Rat
    • SwissProt: Q96CS3 Human
    • SwissProt: Q3TDN2 Mouse
    • SwissProt: Q5BK32 Rat
    • Unigene: 484242 Human
    • Unigene: 194459 Mouse
    • Unigene: 162188 Rat
    see all
  • Alternative names

    • AI462440 antibody
    • expressed in T cells and eosinophils in atopic dermatitis antibody
    • faf2 antibody
    • FAF2_HUMAN antibody
    • Fas associated factor family member 2 antibody
    • FAS-associated factor 2 antibody
    • KIAA0887 antibody
    • Protein ETEA antibody
    • Protein expressed in T cells and eosinophils in atopic dermatitis antibody
    • Protein expressed in T-cells and eosinophils in atopic dermatitis antibody
    • UBX domain containing 8 antibody
    • UBX domain protein 3B antibody
    • UBX domain-containing protein 3B antibody
    • UBX domain-containing protein 8 antibody
    • UBXD8 antibody
    • UBXN3B antibody
    see all

Images

  • Western blot - Anti-UBXD8 antibody (ab154064)
    Western blot - Anti-UBXD8 antibody (ab154064)
    Anti-UBXD8 antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)
    Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)

    Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)

    Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154064? Please let us know so that we can cite the reference in this datasheet.

ab154064 has been referenced in 1 publication.

  • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237

Customer reviews and Q&As

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Western blot abreview for Anti-ETEA antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2, MCF-7)
Specification
HepG2, MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

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Submitted Apr 03 2014

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