Anti-UBXD8 antibody (ab154064)

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Western blot - Anti-UBXD8 antibody (ab154064)
  • Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)

Key features and details

  • Rabbit polyclonal to UBXD8
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-UBXD8 antibody
    See all UBXD8 primary antibodies

  • Description

    Rabbit polyclonal to UBXD8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human UBXD8 aa 176-393.

  • Positive control

    • 293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue

  • General notes

     This product was previously labelled as ETEA

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.

  • Tissue specificity

    Broadly expressed, with highest levels in brain.

  • Sequence similarities

    Contains 1 UBA domain.
    Contains 1 UBX domain.

  • Cellular localization

    Cytoplasm. Lipid droplet. Endoplasmic reticulum.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AI462440 antibody
    • expressed in T cells and eosinophils in atopic dermatitis antibody
    • faf2 antibody
    • FAF2_HUMAN antibody
    • Fas associated factor family member 2 antibody
    • FAS-associated factor 2 antibody
    • KIAA0887 antibody
    • Protein ETEA antibody
    • Protein expressed in T cells and eosinophils in atopic dermatitis antibody
    • Protein expressed in T-cells and eosinophils in atopic dermatitis antibody
    • UBX domain containing 8 antibody
    • UBX domain protein 3B antibody
    • UBX domain-containing protein 3B antibody
    • UBX domain-containing protein 8 antibody
    • UBXD8 antibody
    • UBXN3B antibody
    see all

Images

  • Western blot - Anti-UBXD8 antibody (ab154064)
    Western blot - Anti-UBXD8 antibody (ab154064)
    Anti-UBXD8 antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)
    Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (ab154064)

    Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (ab154064)

    Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.

References (1)

Publishing research using ab154064? Please let us know so that we can cite the reference in this datasheet.

ab154064 has been referenced in 1 publication.

  • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237

Customer reviews and Q&As

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Western blot abreview for Anti-ETEA antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2, MCF-7)
Specification
HepG2, MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Apr 03 2014

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