Overview

  • Product name

  • Description

    Rabbit polyclonal to UBXD8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human UBXD8 aa 176-393.

  • Positive control

    • 293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue
  • General notes

     This product was previously labelled as ETEA

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • Tissue specificity

    Broadly expressed, with highest levels in brain.
  • Sequence similarities

    Contains 1 UBA domain.
    Contains 1 UBX domain.
  • Cellular localization

    Cytoplasm. Lipid droplet. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI462440 antibody
    • expressed in T cells and eosinophils in atopic dermatitis antibody
    • faf2 antibody
    • FAF2_HUMAN antibody
    • Fas associated factor family member 2 antibody
    • FAS-associated factor 2 antibody
    • KIAA0887 antibody
    • Protein ETEA antibody
    • Protein expressed in T cells and eosinophils in atopic dermatitis antibody
    • Protein expressed in T-cells and eosinophils in atopic dermatitis antibody
    • UBX domain containing 8 antibody
    • UBX domain protein 3B antibody
    • UBX domain-containing protein 3B antibody
    • UBX domain-containing protein 8 antibody
    • UBXD8 antibody
    • UBXN3B antibody
    see all

Images

  • Anti-UBXD8 antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.

  • Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.

References

This product has been referenced in:

  • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). Read more (PubMed: 28985237) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2, MCF-7)
Specification
HepG2, MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 03 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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