Anti-UBXD8 antibody (ab154064)
Key features and details
- Rabbit polyclonal to UBXD8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBXD8 antibody
See all UBXD8 primary antibodies -
Description
Rabbit polyclonal to UBXD8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human UBXD8 aa 176-393.
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Positive control
- 293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. -
Tissue specificity
Broadly expressed, with highest levels in brain. -
Sequence similarities
Contains 1 UBA domain.
Contains 1 UBX domain. -
Cellular localization
Cytoplasm. Lipid droplet. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 767948 Cow
- Entrez Gene: 23197 Human
- Entrez Gene: 76577 Mouse
- Entrez Gene: 291000 Rat
- SwissProt: Q96CS3 Human
- SwissProt: Q3TDN2 Mouse
- SwissProt: Q5BK32 Rat
- Unigene: 484242 Human
see all -
Alternative names
- AI462440 antibody
- expressed in T cells and eosinophils in atopic dermatitis antibody
- faf2 antibody
see all
Images
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Anti-UBXD8 antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.
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Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154064 has been referenced in 2 publications.
- Volkmar N et al. Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2. EMBO J 41:e110777 (2022). PubMed: 35993436
- Wang T et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237