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    ubxn1-antibody-epr9136-ab151723.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin
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RecombinantRabMAb

Recombinant Anti-UBXN1 antibody [EPR9136] (ab151723)

  • Datasheet
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Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
  • Anti-UBXN1 antibody [EPR9136] (ab151723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9136] to UBXN1
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-UBXN1 antibody [EPR9136]
    See all UBXN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9136] to UBXN1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human UBXN1.
    Database link: Q04323

  • Positive control

    • HeLa, HepG2, 293T and Ramos cell lysates; Human prostate and testis tissues; 293T cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as LOC51035

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9136
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Alternative Versions

    • Anti-UBXN1 antibody [EPR9136] - BSA and Azide free (ab249041)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist.
    • Cellular localization

      Cytoplasm
    • Database links

      • Entrez Gene: 51035 Human
      • Entrez Gene: 293719 Rat
      • SwissProt: Q04323 Human
      • SwissProt: Q499N6 Rat
      • Unigene: 351296 Human
      • Unigene: 198539 Rat
      • Alternative names

        • 2B28 antibody
        • 4930455J02Rik antibody
        • D19Ertd721e antibody
        • MGC124706 antibody
        • MGC6696 antibody
        • mY33K antibody
        • Protein 2B28 antibody
        • RGD1309471 antibody
        • SAKS1 antibody
        • SAPK substrate protein 1 antibody
        • T25529 antibody
        • UBA/UBX 33.3 kDa protein antibody
        • UBX domain containing protein 1 antibody
        • UBX domain protein 1 antibody
        • UBXD10 antibody
        • UBXN1 antibody
        see all

      Images

      • Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)
        All lanes : Anti-UBXN1 antibody [EPR9136] (ab151723) at 1/10000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : 293T cell lysate
        Lane 4 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 33 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling UBXN1 with ab151723 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling UBXN1 with ab151723 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Flow cytometry analysis of permeabilized 293T cells labeling UBXN1 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

        Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-UBXN1 antibody [EPR9136] (ab151723)
        Anti-UBXN1 antibody [EPR9136] (ab151723)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab151723? Please let us know so that we can cite the reference in this datasheet.

      ab151723 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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