Recombinant Anti-UBXN1 antibody [EPR9136] (ab151723)

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Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9136] to UBXN1
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Human

Product name

Anti-UBXN1 antibody [EPR9136]
See all UBXN1 primary antibodies
Description

Rabbit monoclonal [EPR9136] to UBXN1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human UBXN1.
Database link: Q04323
Positive control
- HeLa, HepG2, 293T and Ramos cell lysates; Human prostate and testis tissues; 293T cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product was previously labelled as LOC51035

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9136
Isotype

IgG
Research areas

Alternative Versions
- Anti-UBXN1 antibody [EPR9136] - BSA and Azide free (ab249041)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human
All applications	Rat

Application	Abreviews	Notes
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
Flow Cyt 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Relevance

Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist.
Cellular localization

Cytoplasm
Database links
- Entrez Gene: 51035 Human
- Entrez Gene: 293719 Rat
- SwissProt: Q04323 Human
- SwissProt: Q499N6 Rat
- Unigene: 351296 Human
- Unigene: 198539 Rat
Alternative names
- 2B28 antibody
- 4930455J02Rik antibody
- D19Ertd721e antibody
- MGC124706 antibody
- MGC6696 antibody
- mY33K antibody
- Protein 2B28 antibody
- RGD1309471 antibody
- SAKS1 antibody
- SAPK substrate protein 1 antibody
- T25529 antibody
- UBA/UBX 33.3 kDa protein antibody
- UBX domain containing protein 1 antibody
- UBX domain protein 1 antibody
- UBXD10 antibody
- UBXN1 antibody
see all

Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)

All lanes : Anti-UBXN1 antibody [EPR9136] (ab151723) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Ramos cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling UBXN1 with ab151723 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling UBXN1 with ab151723 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)

Flow cytometry analysis of permeabilized 293T cells labeling UBXN1 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-UBXN1 antibody [EPR9136] (ab151723)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab151723? Please let us know so that we can cite the reference in this datasheet.

ab151723 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-UBXN1 antibody [EPR9136] (ab151723)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As