Recombinant Anti-UBXN1 antibody [EPR9136] (ab151723)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-UBXN1 antibody [EPR9136]
See all UBXN1 primary antibodies -
Description
Rabbit monoclonal [EPR9136] to UBXN1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human UBXN1.
Database link: Q04323 -
Positive control
- HeLa, HepG2, 293T and Ramos cell lysates; Human prostate and testis tissues; 293T cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as LOC51035
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9136 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/10000 - 1/50000. Predicted molecular weight: 33 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Relevance
Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist. -
Cellular localization
Cytoplasm -
Database links
- Entrez Gene: 51035 Human
- Entrez Gene: 293719 Rat
- SwissProt: Q04323 Human
- SwissProt: Q499N6 Rat
- Unigene: 351296 Human
- Unigene: 198539 Rat
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Alternative names
- 2B28 antibody
- 4930455J02Rik antibody
- D19Ertd721e antibody
see all
Images
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![Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-1.jpg)
Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723)All lanes : Anti-UBXN1 antibody [EPR9136] (ab151723) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling UBXN1 with ab151723 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling UBXN1 with ab151723 at 1/50 dilution.
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![Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-4.jpg)
Flow Cytometry - Anti-UBXN1 antibody [EPR9136] (ab151723)Flow cytometry analysis of permeabilized 293T cells labeling UBXN1 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)](https://www.abcam.com/ps/products/151/ab151723/Images/LOC51035-Primary-antibodies-ab151723-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.
Protocols
Datasheets and documents
References
ab151723 has not yet been referenced specifically in any publications.
