Recombinant Anti-UBXN1 antibody [EPR9136] (ab151723)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9136] to UBXN1
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-UBXN1 antibody [EPR9136]
See all UBXN1 primary antibodies -
Description
Rabbit monoclonal [EPR9136] to UBXN1 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human UBXN1.
Database link: Q04323 -
Positive control
- HeLa, HepG2, 293T and Ramos cell lysates; Human prostate and testis tissues; 293T cells.
-
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as LOC51035
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9136 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
IHC-P |
Human
|
WB |
Human
|
All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Relevance
Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist. -
Cellular localization
Cytoplasm -
Database links
- Entrez Gene: 51035 Human
- Entrez Gene: 293719 Rat
- SwissProt: Q04323 Human
- SwissProt: Q499N6 Rat
- Unigene: 351296 Human
- Unigene: 198539 Rat
-
Alternative names
- 2B28 antibody
- 4930455J02Rik antibody
- D19Ertd721e antibody
see all
Images
-
All lanes : Anti-UBXN1 antibody [EPR9136] (ab151723) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling UBXN1 with ab151723 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling UBXN1 with ab151723 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Flow cytometry analysis of permeabilized 293T cells labeling UBXN1 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (ab151723)
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab151723 has not yet been referenced specifically in any publications.