Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)

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Overview

  • Product name

    Anti-UBXN6/UBXD1 antibody [2F8-24]
    See all UBXN6/UBXD1 primary antibodies

  • Description

    Mouse monoclonal [2F8-24] to UBXN6/UBXD1

  • Host species

    Mouse

  • Specificity

    Only detects the full-length isoform.

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human UBXN6/UBXD1.

  • Positive control

    • U20S cell lysate

  • General notes

     This product was previously labelled as UBXN6

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, 0.5% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    2F8-24

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
IP Use at 1-2 - 0.5 µg/mg of lysate.
ICC/IF Use a concentration of 0.05 - 0.5 µg/ml.

Target

  • Function

    Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.

  • Tissue specificity

    Enhanced expression in testis.

  • Sequence similarities

    Contains 1 PUB (PUG) domain.
    Contains 1 UBX domain.

  • Domain

    The UBX domain lacks key residues critical for VCP binding.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
  • Information by UniProt

  • Database links

    • Alternative names

      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1 µg/ml

      Lane 1 : U20S cells transfected with UBXN6/UBXD1 shRNA
      Lane 2 : U20S cells transfected with mismatch control shRNA

      Predicted band size: 50 kDa
      Observed band size: 54 kDa
      why is the actual band size different from the predicted?

    References

    ab81555 has not yet been referenced specifically in any publications.

    Publishing research using ab81555? Please let us know so that we can cite the reference in this datasheet.

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