Validated using a knockout cell line

Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)

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Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
  • Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)

Key features and details

  • Mouse monoclonal [2F8-24] to UBXN6/UBXD1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-UBXN6/UBXD1 antibody [2F8-24]
    See all UBXN6/UBXD1 primary antibodies

  • Description

    Mouse monoclonal [2F8-24] to UBXN6/UBXD1

  • Host species

    Mouse

  • Specificity

    Only detects the full-length isoform.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human UBXN6/UBXD1.

  • Positive control

    • WB: HEK293T, HeLa and U20S cell lysate

  • General notes

     This product was previously labelled as UBXN6

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, 0.5% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    2F8-24

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function

    Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.

  • Tissue specificity

    Enhanced expression in testis.

  • Sequence similarities

    Contains 1 PUB (PUG) domain.
    Contains 1 UBX domain.

  • Domain

    The UBX domain lacks key residues critical for VCP binding.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
  • Information by UniProt

  • Database links

    • Alternative names

      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1/500 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : UBXN6 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab81555 observed at 50 kDa. Red - loading control ab181602 observed at 36 kDa.

       ab81555 Anti-UBXN6/UBXD1 antibody [2F8-24] was shown to specifically react with UBXN6/UBXD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266689 (knockout cell lysate ab258747) was used. Wild-type and UBXN6/UBXD1 knockout samples were subjected to SDS-PAGE. ab81555 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
      All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1 µg/ml

      Lane 1 : U20S cells transfected with UBXN6/UBXD1 shRNA
      Lane 2 : U20S cells transfected with mismatch control shRNA

      Predicted band size: 50 kDa
      Observed band size: 54 kDa why is the actual band size different from the predicted?

    References (0)

    Publishing research using ab81555? Please let us know so that we can cite the reference in this datasheet.

    ab81555 has not yet been referenced specifically in any publications.

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