Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
Key features and details
- Mouse monoclonal [2F8-24] to UBXN6/UBXD1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-UBXN6/UBXD1 antibody [2F8-24]
See all UBXN6/UBXD1 primary antibodies -
Description
Mouse monoclonal [2F8-24] to UBXN6/UBXD1 -
Host species
Mouse -
Specificity
Only detects the full-length isoform. -
Tested Applications & Species
Application Species WB Human -
Immunogen
Recombinant full length protein corresponding to Human UBXN6/UBXD1.
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Positive control
- WB: HEK293T, HeLa and U20S cell lysate
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General notes
This product was previously labelled as UBXN6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 0.5% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F8-24 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
Target
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Function
Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation. -
Tissue specificity
Enhanced expression in testis. -
Sequence similarities
Contains 1 PUB (PUG) domain.
Contains 1 UBX domain. -
Domain
The UBX domain lacks key residues critical for VCP binding. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 80700 Human
- Omim: 611946 Human
- SwissProt: Q9BZV1 Human
- Unigene: 435255 Human
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Alternative names
- UBX domain containing 1 antibody
- UBX domain containing 2 antibody
- UBX domain containing protein 1 antibody
see all
Images
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All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : UBXN6 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaLanes 1-3: Merged signal (red and green). Green - ab81555 observed at 50 kDa. Red - loading control ab181602 observed at 36 kDa.
ab81555 Anti-UBXN6/UBXD1 antibody [2F8-24] was shown to specifically react with UBXN6/UBXD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266689 (knockout cell lysate ab258747) was used. Wild-type and UBXN6/UBXD1 knockout samples were subjected to SDS-PAGE. ab81555 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1 µg/ml
Lane 1 : U20S cells transfected with UBXN6/UBXD1 shRNA
Lane 2 : U20S cells transfected with mismatch control shRNA
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab81555 has not yet been referenced specifically in any publications.