Overview

  • Product name
  • Description
    Rabbit polyclonal to UCH37
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human UCH37 aa 150-200. The exact sequence is proprietary.
    Database link: NP_057068.1

  • Positive control
    • 293T, Hela, Jurkat, mouse NIH 3T3 and mouse TCMK-1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.

Target

Images

  • All lanes : Anti-UCH37 antibody (ab176377) at 0.1 µg/ml

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : TCMK-1 cell lysate
    Lane 5 : NIH 3T3 cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 30 seconds
  • Detection of UCH37 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with exposure time of seconds.

References

ab176377 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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