Overview

  • Product name

    Anti-UCH37 antibody [EP4897]
    See all UCH37 primary antibodies
  • Description

    Rabbit monoclonal [EP4897] to UCH37
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UCH37 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • 293T, Fetal kidney, JAR, SH-SY5Y and NCCIT lysates; Human kidney tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
    • Sequence similarities

      Belongs to the peptidase C12 family.
    • Cellular localization

      Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • UCH37 antibody
      • AD-019 antibody
      • CGI 70 antibody
      • INO80 complex subunit R antibody
      • INO80R antibody
      • Ubiquitin C terminal hydrolase L5 antibody
      • Ubiquitin C terminal hydrolase UCH37 antibody
      • Ubiquitin C-terminal hydrolase UCH37 antibody
      • Ubiquitin carboxyl terminal esterase L5 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L5 antibody
      • Ubiquitin carboxyl terminal hydrolase L5 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L5 antibody
      • Ubiquitin thioesterase L5 antibody
      • Ubiquitin thiolesterase L5 antibody
      • UCH L5 antibody
      • UCH-L5 antibody
      • UCHL5 antibody
      • UCHL5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-UCH37 antibody [EP4897] (ab133508) at 1/10000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Jar lysate
      Lane 4 : SH-SY5Y lysate
      Lane 5 : NCCIT lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling UCH37 with ab133508 at 1/100 dilution.

    References

    This product has been referenced in:

    • Shruthi K  et al. Ubiquitin-proteasome system and ER stress in the brain of diabetic rats. J Cell Biochem 120:5962-5973 (2019). Read more (PubMed: 30317658) »
    • Reddy SS  et al. 4-PBA prevents diabetic muscle atrophy in rats by modulating ER stress response and ubiquitin-proteasome system. Chem Biol Interact 306:70-77 (2019). Read more (PubMed: 30980806) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133508.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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