Product nameAnti-UCH37 antibody [EPR4896]
See all UCH37 primary antibodies
DescriptionRabbit monoclonal [EPR4896] to UCH37
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC
Species reactivityReacts with: Rat, Human
Does not react with: Mouse
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
- Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProtease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
Sequence similaritiesBelongs to the peptidase C12 family.
Cellular localizationCytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
- Information by UniProt
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysates
Lane 2 : 293T cell lysates
Lane 3 : NCCIT cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Liao Y et al. USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discov 5:24 (2019). Read more (PubMed: 31044085) »
- Sha B et al. Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma. Apoptosis 24:826-836 (2019). Read more (PubMed: 31342239) »