Recombinant
RabMAb

Recombinant Anti-UCH37 antibody [EPR4896] (Alexa Fluor® 488) (ab223411)

Overview

  • Product name

    Anti-UCH37 antibody [EPR4896] (Alexa Fluor® 488)
    See all UCH37 primary antibodies
  • Description

    Rabbit monoclonal [EPR4896] to UCH37 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Human UCH37 aa 1-100.
    Database link: Q9Y5K5

  • Positive control

    • IHC-Fr: normal rat kidney tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.

Target

  • Function

    Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Cellular localization

    Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • UCH37 antibody
    • AD-019 antibody
    • CGI 70 antibody
    • INO80 complex subunit R antibody
    • INO80R antibody
    • Ubiquitin C terminal hydrolase L5 antibody
    • Ubiquitin C terminal hydrolase UCH37 antibody
    • Ubiquitin C-terminal hydrolase UCH37 antibody
    • Ubiquitin carboxyl terminal esterase L5 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L5 antibody
    • Ubiquitin carboxyl terminal hydrolase L5 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L5 antibody
    • Ubiquitin thioesterase L5 antibody
    • Ubiquitin thiolesterase L5 antibody
    • UCH L5 antibody
    • UCH-L5 antibody
    • UCHL5 antibody
    • UCHL5_HUMAN antibody
    see all

Images

  • IHC image of UCH37 staining in a section of frozen normal rat kidney.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab223411 at 1/1000 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

References

ab223411 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223411.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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