Anti-UCHL3 antibody (ab244371)
Key features and details
- Rabbit polyclonal to UCHL3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UCHL3 antibody
See all UCHL3 primary antibodies -
Description
Rabbit polyclonal to UCHL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human UCHL3 aa 1-100.
Database link: P15374 -
Positive control
- IHC-P: Human testis, colon, rectum and prostate tissue. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. -
Tissue specificity
Highly expressed in heart, skeletal muscle, and testis. -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 520170 Cow
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 768115 Pig
- Entrez Gene: 498560 Rat
- Omim: 603090 Human
- SwissProt: Q2TBG8 Cow
- SwissProt: P15374 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for UCHL3 (green) using ab244371 at 4 µg/ml in ICC/IF.
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All lanes : Anti-UCHL3 antibody (ab244371) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Paraffin-embedded human testis tissue stained for UCHL3 using ab244371 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for UCHL3 using ab244371 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human rectum tissue stained for UCHL3 using ab244371 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon tissue stained for UCHL3 using ab244371 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for UCHL3 using ab244371 at 1/50 dilution in immunohistochemical analysis.
No positivity in exocrine glandular cells as expected.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab244371 has been referenced in 1 publication.
- Kang JW et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736