Recombinant Anti-UCHL3 antibody [EP5331] (ab126621)
![Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/ab126621-394822-anti-uchl3-antibody-ep5331-westernblot-human-mouse-rat.jpg "Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5331] to UCHL3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-UCHL3 antibody [EP5331]
See all UCHL3 primary antibodies -
Description
Rabbit monoclonal [EP5331] to UCHL3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human UCHL3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P15374 -
Positive control
- WB: K562, 293T, SH-SY5Y and Jurkat cell lysates. Mouse and rat brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.27 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP5331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
For unpurified use at 1/1000 - 1/10000 |
| Notes |
|---|
|
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). For unpurified use at 1/1000 - 1/10000 |
Target
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Function
Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. -
Tissue specificity
Highly expressed in heart, skeletal muscle, and testis. -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 498560 Rat
- Omim: 603090 Human
- SwissProt: P15374 Human
- SwissProt: Q9JKB1 Mouse
- SwissProt: Q91Y78 Rat
- Unigene: 162241 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
-
Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)All lanes : Anti-UCHL3 antibody [EP5331] (ab126621) at 1/10000 dilution (Purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/UCHL3-Primary-antibodies-ab126621-1.jpg)
Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)All lanes : Anti-UCHL3 antibody [EP5331] (ab126621) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
![OI-RD Scanning - Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/UCHL3-Primary-antibodies-ab126621-2.jpg)
OI-RD Scanning - Anti-UCHL3 antibody [EP5331] (ab126621) -
![Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/ab126621-4-benefits-of-recombinant-antibodies.png)
Anti-UCHL3 antibody [EP5331] (ab126621)
Datasheets and documents
References (0)
ab126621 has not yet been referenced specifically in any publications.
