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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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RecombinantRabMAb

Recombinant Anti-UCHL3 antibody [EP5331] (ab126621)

  • Datasheet
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Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
  • Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
  • OI-RD Scanning - Anti-UCHL3 antibody [EP5331] (ab126621)
  • Anti-UCHL3 antibody [EP5331] (ab126621)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP5331] to UCHL3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant human UCHL3 protein (ab103502)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-UCHL3 antibody [EP5331]
    See all UCHL3 primary antibodies
  • Description

    Rabbit monoclonal [EP5331] to UCHL3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human UCHL3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P15374

  • Positive control

    • WB: K562, 293T, SH-SY5Y and Jurkat cell lysates. Mouse and rat brain tissue lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 5.27 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP5331
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Alternative Versions

    • Anti-UCHL3 antibody [EP5331] - BSA and Azide free (ab248130)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human UCHL3 protein (ab103502)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (1)
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

For unpurified use at 1/1000 - 1/10000

Notes
WB
1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

For unpurified use at 1/1000 - 1/10000

Target

  • Function

    Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
  • Tissue specificity

    Highly expressed in heart, skeletal muscle, and testis.
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P15374 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7347 Human
    • Entrez Gene: 50933 Mouse
    • Entrez Gene: 498560 Rat
    • Omim: 603090 Human
    • SwissProt: P15374 Human
    • SwissProt: Q9JKB1 Mouse
    • SwissProt: Q91Y78 Rat
    • Unigene: 162241 Human
    • Unigene: 275970 Mouse
    • Unigene: 145487 Rat
    see all
  • Alternative names

    • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
    • Ubiquitin thioesterase L3 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L3 antibody
    • UCH L3 antibody
    • UCH-L3 antibody
    • UCHL3 antibody
    • UCHL3_HUMAN antibody
    see all

Images

  • Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
    Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
    All lanes : Anti-UCHL3 antibody [EP5331] (ab126621) at 1/10000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
    Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)
    All lanes : Anti-UCHL3 antibody [EP5331] (ab126621) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • OI-RD Scanning - Anti-UCHL3 antibody [EP5331] (ab126621)
    OI-RD Scanning - Anti-UCHL3 antibody [EP5331] (ab126621)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-UCHL3 antibody [EP5331] (ab126621)
    Anti-UCHL3 antibody [EP5331] (ab126621)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab126621? Please let us know so that we can cite the reference in this datasheet.

    ab126621 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-UCHL3 antibody [EP5331]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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