- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-UCHL3 antibody [EP5331]
See all UCHL3 primary antibodies -
DescriptionRabbit monoclonal [EP5331] to UCHL3
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human UCHL3 (N terminal). The exact sequence is proprietary.
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Positive control
- K562, 293T, SH-SY5Y and Jurkat cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 5.27 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP5331
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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FunctionDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
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Tissue specificityHighly expressed in heart, skeletal muscle, and testis.
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Sequence similaritiesBelongs to the peptidase C12 family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 498560 Rat
- Omim: 603090 Human
- SwissProt: P15374 Human
- SwissProt: Q9JKB1 Mouse
- SwissProt: Q91Y78 Rat
- Unigene: 162241 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
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![Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/UCHL3-Primary-antibodies-ab126621-1.jpg)
Western blot - Anti-UCHL3 antibody [EP5331] (ab126621)All lanes : Anti-UCHL3 antibody [EP5331] (ab126621) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
![Other - Anti-UCHL3 antibody [EP5331] (ab126621)](https://www.abcam.com/ps/products/126/ab126621/Images/UCHL3-Primary-antibodies-ab126621-2.jpg)
Other - Anti-UCHL3 antibody [EP5331] (ab126621)
Protocols
References
ab126621 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"