Recombinant Anti-UCHL3 antibody [EPR5332] (ab126703)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)](https://www.abcam.com/ps/products/126/ab126703/Images/ab126703-140642-anti-uchl3-antibody-epr5332-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5332] to UCHL3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-UCHL3 antibody [EPR5332]
See all UCHL3 primary antibodies -
Description
Rabbit monoclonal [EPR5332] to UCHL3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human UCHL3 aa 200-300 (C terminal). The exact sequence is proprietary.
-
Positive control
- 293T, SH-SY5Y, Jurkat, and K562 cell lysates; Human testis tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5332 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. -
Tissue specificity
Highly expressed in heart, skeletal muscle, and testis. -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 498560 Rat
- Omim: 603090 Human
- SwissProt: P15374 Human
- SwissProt: Q9JKB1 Mouse
- SwissProt: Q91Y78 Rat
- Unigene: 162241 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)
ab126703, at 1/100, staining UCHL3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)](https://www.abcam.com/ps/products/126/ab126703/Images/ab126703-140641-anti-uchl3-antibody-epr5332-western-blot.jpg)
Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)All lanes : Anti-UCHL3 antibody [EPR5332] (ab126703) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Anti-UCHL3 antibody [EPR5332] (ab126703)](https://www.abcam.com/ps/products/126/ab126703/Images/ab126703-3-benefits-of-recombinant-antibodies.png)
Anti-UCHL3 antibody [EPR5332] (ab126703)
Protocols
Datasheets and documents
References (3)
ab126703 has been referenced in 3 publications.
- Ouyang L et al. The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor. Signal Transduct Target Ther 5:78 (2020). PubMed: 32546741
- Olie CS et al. Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting. iScience 12:318-332 (2019). PubMed: 30739015
- Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . PubMed: 26934049
