Recombinant
RabMAb

Recombinant Anti-UCHL3 antibody [EPR5332] (ab126703)

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Overview

  • Product name

    Anti-UCHL3 antibody [EPR5332]
    See all UCHL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5332] to UCHL3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human UCHL3 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, SH-SY5Y, Jurkat, and K562 cell lysates; Human testis tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab126703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.

    • Tissue specificity

      Highly expressed in heart, skeletal muscle, and testis.

    • Sequence similarities

      Belongs to the peptidase C12 family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
      • Ubiquitin thioesterase L3 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L3 antibody
      • UCH L3 antibody
      • UCH-L3 antibody
      • UCHL3 antibody
      • UCHL3_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)

      ab126703, at 1/100, staining UCHL3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)
      Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)
      All lanes : Anti-UCHL3 antibody [EPR5332] (ab126703) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    References

    This product has been referenced in:

    • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »
    See 1 Publication for this product

    Publishing research using ab126703? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-UCHL3 antibody [EPR5332]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

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    Verified customer

    Submitted Oct 10 2016

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