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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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RecombinantRabMAb

Recombinant Anti-UCHL3 antibody [EPR5332] (ab126703)

  • Datasheet
Reviews (1) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)
  • Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)
  • Anti-UCHL3 antibody [EPR5332] (ab126703)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5332] to UCHL3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Protein
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Recombinant human UCHL3 protein (ab103502)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-UCHL3 antibody [EPR5332]
    See all UCHL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5332] to UCHL3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human UCHL3 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, SH-SY5Y, Jurkat, and K562 cell lysates; Human testis tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5332
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Alternative Versions

    • Anti-UCHL3 antibody [EPR5332] - BSA and Azide free (ab248136)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant human UCHL3 protein (ab103502)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
    • Tissue specificity

      Highly expressed in heart, skeletal muscle, and testis.
    • Sequence similarities

      Belongs to the peptidase C12 family.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P15374 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7347 Human
      • Entrez Gene: 50933 Mouse
      • Entrez Gene: 498560 Rat
      • Omim: 603090 Human
      • SwissProt: P15374 Human
      • SwissProt: Q9JKB1 Mouse
      • SwissProt: Q91Y78 Rat
      • Unigene: 162241 Human
      • Unigene: 275970 Mouse
      • Unigene: 145487 Rat
      see all
    • Alternative names

      • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
      • Ubiquitin thioesterase L3 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L3 antibody
      • UCH L3 antibody
      • UCH-L3 antibody
      • UCHL3 antibody
      • UCHL3_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)

      ab126703, at 1/100, staining UCHL3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)
      Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)
      All lanes : Anti-UCHL3 antibody [EPR5332] (ab126703) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Anti-UCHL3 antibody [EPR5332] (ab126703)
      Anti-UCHL3 antibody [EPR5332] (ab126703)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab126703? Please let us know so that we can cite the reference in this datasheet.

    ab126703 has been referenced in 3 publications.

    • Ouyang L  et al. The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor. Signal Transduct Target Ther 5:78 (2020). PubMed: 32546741
    • Olie CS  et al. Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting. iScience 12:318-332 (2019). PubMed: 30739015
    • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . PubMed: 26934049

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-UCHL3 antibody [EPR5332]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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