Recombinant
Anti-UCHL3 antibody [EPR5332] (ab126703)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-UCHL3 antibody [EPR5332]
See all UCHL3 primary antibodies -
DescriptionRabbit monoclonal [EPR5332] to UCHL3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human UCHL3 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, SH-SY5Y, Jurkat, and K562 cell lysates; Human testis tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR5332
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | 1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,ICC or IP.
Target
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FunctionDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
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Tissue specificityHighly expressed in heart, skeletal muscle, and testis.
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Sequence similaritiesBelongs to the peptidase C12 family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 498560 Rat
- Omim: 603090 Human
- SwissProt: P15374 Human
- SwissProt: Q9JKB1 Mouse
- SwissProt: Q91Y78 Rat
- Unigene: 162241 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)](https://www.abcam.com/ps/datasheet/images/126/ab126703/UCHL3-Primary-antibodies-ab126703-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody [EPR5332] (ab126703)ab126703, at 1/100, staining UCHL3 in Paraffin-embedded Human testis tissue by Immunohistochemistry. -
![Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)](https://www.abcam.com/ps/datasheet/images/126/ab126703/UCHL3-Primary-antibodies-ab126703-2.jpg)
Western blot - Anti-UCHL3 antibody [EPR5332] (ab126703)All lanes : Anti-UCHL3 antibody [EPR5332] (ab126703) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
References
This product has been referenced in:
- Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"