Recombinant
RabMAb

Recombinant Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

Overview

  • Product name

    Anti-UCHL5IP antibody [EPR15341-56]
    See all UCHL5IP primary antibodies
  • Description

    Rabbit monoclonal [EPR15341-56] to UCHL5IP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human UCHL5IP aa 150-300. The exact sequence is proprietary.
    Database link: Q99871

  • Positive control

    • HeLa, Jurkat and RAW 264.7 cell lysates; Human endometrium adenocarcinoma, Human liver and Mouse kidney tissues; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15341-56
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/480.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/150.

Target

  • Function

    Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
  • Tissue specificity

    Detected in spleen, thymus, testis, ovary, small intestine and colon, with highest levels of expression in testis and ovary.
  • Sequence similarities

    Belongs to the HAUS7 family.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Localizes to interphase centrosomes and to mitotic spindle microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome-associated UCH interacting protein 1 antibody
    • 26S proteasome-associated UCH37-interacting protein 1 antibody
    • Expressed-Xq28STS protein antibody
    • HAUS augmin-like complex subunit 7 antibody
    • Haus7 antibody
    • HAUS7_HUMAN antibody
    • HSXQ28ORF antibody
    • UCH37 interacting protein 1 antibody
    • UCHL5 interacting protein antibody
    • UCHL5-interacting protein antibody
    • UCHL5IP antibody
    • UIP1 antibody
    • X-linked protein STS1769 antibody
    see all

Images

  • All lanes : Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution + RAW 264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

  • Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab192616 at 1/150 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling UCHL5IP with ab192616 at 1/480 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab192616 has not yet been referenced specifically in any publications.

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