Recombinant
RabMAb

Recombinant Anti-UCKL1 antibody [EPR7384] (ab126754)

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Overview

  • Product name

    Anti-UCKL1 antibody [EPR7384]
    See all UCKL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7384] to UCKL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human UCKL1 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • 293T and HeLa cell lysates; Human kidney tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.30 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7384

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      May contribute to UTP accumulation needed for blast transformation and proliferation.

    • Tissue specificity

      Ubiquitous.

    • Pathway

      Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
      Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.

    • Sequence similarities

      Belongs to the uridine kinase family.

    • Post-translational
      modifications

      Ubiquitinated by RNF19B; which induces proteasomal degradation.

    • Cellular localization

      Cytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location.
    • Information by UniProt

    • Database links

      • Alternative names

        • F538 antibody
        • UCK 1L antibody
        • UCK1 like antibody
        • UCK1L antibody
        • UCKL 1 antibody
        • UCKL1 antibody
        • UCKL1_HUMAN antibody
        • Uridine cytidine kinase 1 like 1 antibody
        • Uridine kinase like 1 antibody
        • Uridine-cytidine kinase-like 1 antibody
        • Uridine/cytidine kinase like 1 antibody
        • URKL 1 antibody
        • URKL1 antibody
        see all

      Images

      • Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754)
        Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754)
        All lanes : Anti-UCKL1 antibody [EPR7384] (ab126754) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

        Predicted band size: 61 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (ab126754)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (ab126754)

        ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Other - Anti-UCKL1 antibody [EPR7384] (ab126754)
        Other - Anti-UCKL1 antibody [EPR7384] (ab126754)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab126754 has not yet been referenced specifically in any publications.

      Publishing research using ab126754? Please let us know so that we can cite the reference in this datasheet.

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