Recombinant Anti-UCKL1 antibody [EPR7384] (ab126754)
![Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754)](https://www.abcam.com/ps/products/126/ab126754/Images/ab126754-140767-anti-uckl1-antibody-epr7384-western-blot.jpg "Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7384] to UCKL1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-UCKL1 antibody [EPR7384]
See all UCKL1 primary antibodies -
Description
Rabbit monoclonal [EPR7384] to UCKL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human UCKL1 aa 500-600. The exact sequence is proprietary.
-
Positive control
- 293T and HeLa cell lysates; Human kidney tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7384 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May contribute to UTP accumulation needed for blast transformation and proliferation. -
Tissue specificity
Ubiquitous. -
Pathway
Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1. -
Sequence similarities
Belongs to the uridine kinase family. -
Post-translational
modificationsUbiquitinated by RNF19B; which induces proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location. - Information by UniProt
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Database links
- Entrez Gene: 54963 Human
- Entrez Gene: 68556 Mouse
- Omim: 610866 Human
- SwissProt: Q9NWZ5 Human
- SwissProt: Q91YL3 Mouse
- Unigene: 504998 Human
- Unigene: 232400 Mouse
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Alternative names
- F538 antibody
- UCK 1L antibody
- UCK1 like antibody
see all
Images
-
Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754)All lanes : Anti-UCKL1 antibody [EPR7384] (ab126754) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (ab126754)](https://www.abcam.com/ps/products/126/ab126754/Images/ab126754-140766-anti-uckl1-antibody-epr7384-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (ab126754)ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] (ab126754)](https://www.abcam.com/ps/products/126/ab126754/Images/ab126754-140765-anti-uckl1-antibody-epr7384.jpg)
OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] (ab126754) -
![Anti-UCKL1 antibody [EPR7384] (ab126754)](https://www.abcam.com/ps/products/126/ab126754/Images/ab126754-4-benefits-of-recombinant-antibodies.png)
Anti-UCKL1 antibody [EPR7384] (ab126754)
Protocols
Datasheets and documents
References (0)
ab126754 has not yet been referenced specifically in any publications.
