Overview

  • Product name

    Anti-UCKL1 antibody [EPR7384]
    See all UCKL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7384] to UCKL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UCKL1 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • 293T and HeLa cell lysates; Human kidney tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7384
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May contribute to UTP accumulation needed for blast transformation and proliferation.
    • Tissue specificity

      Ubiquitous.
    • Pathway

      Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
      Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
    • Sequence similarities

      Belongs to the uridine kinase family.
    • Post-translational
      modifications

      Ubiquitinated by RNF19B; which induces proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location.
    • Information by UniProt
    • Database links

    • Alternative names

      • F538 antibody
      • UCK 1L antibody
      • UCK1 like antibody
      • UCK1L antibody
      • UCKL 1 antibody
      • UCKL1 antibody
      • UCKL1_HUMAN antibody
      • Uridine cytidine kinase 1 like 1 antibody
      • Uridine kinase like 1 antibody
      • Uridine-cytidine kinase-like 1 antibody
      • Uridine/cytidine kinase like 1 antibody
      • URKL 1 antibody
      • URKL1 antibody
      see all

    Images

    • All lanes : Anti-UCKL1 antibody [EPR7384] (ab126754) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 61 kDa

    • ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126754 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126754.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up