Overview

  • Product name
  • Description
    Rabbit polyclonal to UCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Spermophilus tridecemlineatus
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 145-159 of Human UCP1, with N-terminal cysteine added.

  • Positive control
    • ICC/IF: Murine fat progenitor cells. IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab10983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. PubMed: 22545021
WB 1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
IHC-P 1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.

Target

  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity
    Brown adipose tissue.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Form
    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • ab10983, staining UCP1 (green), in undifferentiated (left) and differentiated (right) murine fat progenitor cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated with primary antibody at 1/500 dilution. An AlexaFluor®546-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained using DAPI (blue).
  • All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml

    Lane 1 : Rat brown fat
    Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
  • ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin.

References

This product has been referenced in:

See all 210 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (BAT, WAT, Liver, Skeletal Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
BAT, WAT, Liver, Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Marcus .

Verified customer

Submitted Jun 05 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Peromyscus leucopus Tissue sections (Brown adipose tissue, adipocyte)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Brown adipose tissue, adipocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Fixative
Paraformaldehyde
Username

Holly Bates

Verified customer

Submitted Dec 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
20 µg
Specification
brown adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v TritonX-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 06 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (mesenchymal stem cells)
Permeabilization
Yes - Triton 0.25%
Specification
mesenchymal stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (fetal visceral fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
fetal visceral fat
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Username

Miss. Barbara Makela

Verified customer

Submitted Apr 15 2016

Application
Western blot
Sample
Sheep Cell lysate - whole cell (preadipocyte)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% PAG)
Loading amount
10 µg
Specification
preadipocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Miss. Barbara Makela

Verified customer

Submitted Apr 15 2016

Application
Western blot
Sample
Sheep Cell lysate - whole cell (Ovine brown adipocyte precursor cells)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
30 µg
Treatment
9 day induction of differentiation with a serum-free Dulbecco's modified Eagle Ham/F12 medium supplemented with 100 nM dexamethasone, 1 μg/mL insulin, 10 pg/mL transferrin, and 1 nM triiodothyronine
Specification
Ovine brown adipocyte precursor cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brown adipose tissue)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brown adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 27 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization
No
Specification
breast tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

Submitted Aug 17 2015

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