Anti-UCP1 antibody (ab10983)
- Datasheet
- References (255)
- Protocols
Overview
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Product nameAnti-UCP1 antibody
See all UCP1 primary antibodies -
DescriptionRabbit polyclonal to UCP1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Spermophilus tridecemlineatus
Predicted to work with: Sheep, Rabbit, Cow, Dog, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human UCP1 aa 145-159 conjugated to keyhole limpet haemocyanin (Cysteine residue).
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Positive control
- ICC/IF: Murine fat progenitor cells. IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat.
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General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab10983 is affinity purified using the immunogenic peptide immobilized on agarose.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab10983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. PubMed: 22545021 | |
| WB | 1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples. | |
| IHC-P | 1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue. |
Target
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FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
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Tissue specificityBrown adipose tissue.
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Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 281561 Cow
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- SwissProt: P10861 Cow
- SwissProt: P12242 Mouse
- SwissProt: P14271 Rabbit
- SwissProt: P04633 Rat
- Unigene: 4177 Mouse
see all -
FormUCP1 is preferentially expressed in brown adipose tissue
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Alternative names
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 5.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin. -
Immunocytochemistry/ Immunofluorescence - Anti-UCP1 antibody (ab10983)Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 3.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.ab10983, staining UCP1 (green), in undifferentiated (Left panel) and differentiated (Right panel) murine fat progenitor cells, by Immunocytochemistry/ Immunofluorescence.
Cells were incubated with primary antibody at 1/500 dilution.An Alexa-Fluor®546-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
Nuclei were stained using DAPI (blue).
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Western blot - Anti-UCP1 antibody (ab10983)All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml
Lane 1 : Rat brown fat
Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
Protocols
References
This product has been referenced in:
- Fujimoto Y et al. Metabolic changes in adipose tissues in response to ß3 -adrenergic receptor activation in mice. J Cell Biochem 120:821-835 (2019). Read more (PubMed: 30191605) »
- Cunarro J et al. p107 Deficiency Increases Energy Expenditure by Inducing Brown-Fat Thermogenesis and Browning of White Adipose Tissue. Mol Nutr Food Res 63:e1801096 (2019). Read more (PubMed: 30383332) »
