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    ucp1-antibody-ab10983.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-UCP1 antibody (ab10983)

  • Datasheet
  • SDS
Reviews (23)Q&A (10)References (604)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)
  • Western blot - Anti-UCP1 antibody (ab10983)

Key features and details

  • Rabbit polyclonal to UCP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-UCP1 antibody [EPR20381] (ab209483)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-UCP1 antibody
    See all UCP1 primary antibodies
  • Description

    Rabbit polyclonal to UCP1
  • Host species

    Rabbit
  • Specificity

    This polyclonal antibody does not undergo batch testing for mouse western blot, and we cannot therefore guarantee this reagent for this species/application. For an alternative product please see ab209483 which is guaranteed for mouse western blot.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Human, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human UCP1 aa 145-159 conjugated to keyhole limpet haemocyanin (Cysteine residue).

  • Positive control

    • IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat lysate.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab10983 is affinity purified using the immunogenic peptide immobilized on agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human UCP1 protein (ab152782)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (11)
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
IHC-P (8)
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.
Notes
WB
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
IHC-P
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.

Target

  • Function

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity

    Brown adipose tissue.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P25874 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281561 Cow
    • Entrez Gene: 7350 Human
    • Entrez Gene: 22227 Mouse
    • Entrez Gene: 24860 Rat
    • Omim: 113730 Human
    • SwissProt: P10861 Cow
    • SwissProt: P25874 Human
    • SwissProt: P12242 Mouse
    • SwissProt: P14271 Rabbit
    • SwissProt: P04633 Rat
    • Unigene: 249211 Human
    • Unigene: 4177 Mouse
    • Unigene: 10281 Rat
    see all
  • Form

    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names

    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)

    Immunohistochemical analysis of  formalin-fixed, paraffin-embedded mouse brown adipose tissue staining UCP1 using ab10983 at 1/500 dilution. Detected using an Anti-Rabbit IgG-biotin conjugate and an Avidin-Peroxidase conjugate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 5.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.
    ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin.
  • Western blot - Anti-UCP1 antibody (ab10983)
    Western blot - Anti-UCP1 antibody (ab10983)
    All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml

    Lane 1 : Rat brown fat
    Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (604)

Publishing research using ab10983? Please let us know so that we can cite the reference in this datasheet.

ab10983 has been referenced in 604 publications.

  • Han MS  et al. A feed-forward regulatory loop in adipose tissue promotes signaling by the hepatokine FGF21. Genes Dev 35:133-146 (2021). PubMed: 33334822
  • Wilson EA  et al. Gqa/G11a deficiency in dorsomedial hypothalamus leads to obesity resulting from decreased energy expenditure and impaired sympathetic nerve activity. Am J Physiol Endocrinol Metab 320:E270-E280 (2021). PubMed: 33166186
  • Morón-Ros S  et al. FGF15/19 is required for adipose tissue plasticity in response to thermogenic adaptations. Mol Metab 43:101113 (2021). PubMed: 33171307
  • Caputo T  et al. Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue. Cell Mol Life Sci 78:227-247 (2021). PubMed: 32157317
  • Ko MS  et al. Mitophagy deficiency increases NLRP3 to induce brown fat dysfunction in mice. Autophagy 17:1205-1221 (2021). PubMed: 32400277
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 33 Abreviews or Q&A

Western blot abreview for Anti-UCP1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
MEF cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Mar 30 2021

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brown adipocyte tissue)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
10 µg
Specification
Brown adipocyte tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Qixiang Ma

Verified customer

Submitted Aug 04 2020

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20 %)
Loading amount
10 µg
Treatment
leptin
Specification
mouse brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UCP1 antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (BAT, WAT, Liver, Skeletal Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
BAT, WAT, Liver, Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Marcus .

Verified customer

Submitted Jun 05 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Peromyscus leucopus Tissue sections (Brown adipose tissue, adipocyte)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Brown adipose tissue, adipocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Fixative
Paraformaldehyde
Read More

Holly Bates

Verified customer

Submitted Dec 01 2017

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
20 µg
Specification
brown adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Sep 28 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v TritonX-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Feb 06 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (mesenchymal stem cells)
Permeabilization
Yes - Triton 0.25%
Specification
mesenchymal stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 04 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UCP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (fetal visceral fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
fetal visceral fat
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Read More

MISS. Barbara Makela

Verified customer

Submitted Apr 15 2016

Western blot abreview for Anti-UCP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Sheep Cell lysate - whole cell (preadipocyte)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% PAG)
Loading amount
10 µg
Specification
preadipocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

MISS. Barbara Makela

Verified customer

Submitted Apr 15 2016

1-10 of 33 Abreviews or Q&A

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