Mouse, Rat, Spermophilus tridecemlineatus Predicted to work with:
Sheep, Rabbit, Cow, Dog, Macaque monkey
Synthetic peptide conjugated to KLH, corresponding to amino acids 145-159 of Human UCP1, with N-terminal cysteine added.
ICC/IF: Murine fat progenitor cells.
IHC-P: Murine inguinal white adipose tissue.
WB: Rat brown fat.
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. PubMed: 22545021
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
Brown adipose tissue.
Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.