Anti-UCP1 antibody (ab10983)
Key features and details
- Rabbit polyclonal to UCP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-UCP1 antibody
See all UCP1 primary antibodies -
Description
Rabbit polyclonal to UCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Sheep, Rabbit, Cow, Dog, Human, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human UCP1 aa 145-159 conjugated to keyhole limpet haemocyanin (Cysteine residue).
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Positive control
- IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat lysate.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab10983 is affinity purified using the immunogenic peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab10983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples. | |
| IHC-P | 1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue. |
Target
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Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
Tissue specificity
Brown adipose tissue. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 281561 Cow
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P10861 Cow
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
see all -
Form
UCP1 is preferentially expressed in brown adipose tissue -
Alternative names
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)Immunohistochemical analysis of fprmalin-fixed, paraffin-embedded mouse brown adipose tissue staining UCP1 using ab10983 at 1/500 dilution. Detected using an Anti-Rabbit IgG-biotin conjugate and an Avidin-Peroxidase conjugate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab10983)Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 5.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin. -
Western blot - Anti-UCP1 antibody (ab10983)All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml
Lane 1 : Rat brown fat
Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
Protocols
References
ab10983 has been referenced in 428 publications.
- Johnson JM et al. Alternative splicing of UCP1 by non-cell-autonomous action of PEMT. Mol Metab 31:55-66 (2020). PubMed: 31918922
- Badenes M et al. Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis. Mol Metab 31:67-84 (2020). PubMed: 31918923
- Siang DTC et al. The RNA-binding protein HuR is a negative regulator in adipogenesis. Nat Commun 11:213 (2020). PubMed: 31924774
- Kang JG et al. A Mouse Homolog of a Human TP53 Germline Mutation Reveals a Lipolytic Activity of p53. Cell Rep 30:783-792.e5 (2020). PubMed: 31968253
- Sousa-Filho CPB et al. Green tea improves the metabolism of peripheral tissues in ß3-adrenergic receptor-knockout mice. Pharmacol Res 159:104956 (2020). PubMed: 32480000
