Application
Western blot
Sample
Mouse Tissue lysate - whole (brown fat tissue extract)
Loading amount
30 µg
Specification
brown fat tissue extract
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/3000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: rabbit polyclonal antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 32 kDa kDa
DR. Guo-Ping Shi
Verified customer
Submitted Jul 04 2008