Overview

  • Product name

  • Description

    Rabbit polyclonal to UCP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-299 of Human UCP1 (UniProt P25874).

  • Positive control

    • Mouse interscapular brown adipose tissue whole cell lysate, Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.

Target

  • Function

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity

    Brown adipose tissue.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Form

    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names

    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • Anti-UCP1 antibody (ab155117) at 1/1000 dilution + Mouse interscapular brown adipose tissue whole cell lysate at 2.5 µg

    Predicted band size: 33 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, labeling UCP1 with ab155117 at 1/250 dilution.

References

This product has been referenced in:

See all 13 Publications for this product

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