Overview

  • Product name
  • Description
    Rabbit polyclonal to UCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-299 of Human UCP1 (UniProt P25874).

  • Positive control
    • Mouse interscapular brown adipose tissue whole cell lysate, Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.

Target

  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity
    Brown adipose tissue.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Form
    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • Anti-UCP1 antibody (ab155117) at 1/1000 dilution + Mouse interscapular brown adipose tissue whole cell lysate at 2.5 µg

    Predicted band size: 33 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, labeling UCP1 with ab155117 at 1/250 dilution.

References

This product has been referenced in:
  • Wood J  et al. BDNF mimetic alleviates body weight gain in obese mice by enhancing mitochondrial biogenesis in skeletal muscle. Metabolism 87:113-122 (2018). Read more (PubMed: 29935237) »
  • Vargas D  et al. Thermogenic capacity of human periaortic adipose tissue is transformed by body weight. PLoS One 13:e0194269 (2018). Read more (PubMed: 29554135) »
See all 12 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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