- Datasheet
- Specific References (53)
- Protocols
Overview
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Product nameAnti-UCP1 antibody
See all UCP1 primary antibodies -
DescriptionRabbit polyclonal to UCP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Dog
Predicted to work with: Hamster, Human, Chimpanzee
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP1.
(Peptide available as ab24282.)
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Positive control
- WB: Mouse and rat brown adipose tissue lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab23841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/1000. |
Target
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FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
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Tissue specificityBrown adipose tissue.
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Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
- SwissProt: P04633 Rat
- Unigene: 249211 Human
see all -
FormUCP1 is preferentially expressed in brown adipose tissue
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Alternative names
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
Images
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Western blot - Anti-UCP1 antibody (ab23841)All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml
Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
Lane 2 : Adult Rat Brown Adipose Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab23841)Image from Liu W et al., PLoS Genet. 2006;8:11958-63. Fig 5.; doi: 10.1371/journal.pgen.1003626ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.
References
This product has been referenced in:
- Hou X et al. CDK6 inhibits white to beige fat transition by suppressing RUNX1. Nat Commun 9:1023 (2018). Read more (PubMed: 29523786) »
- Ding C et al. De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nat Commun 9:1329 (2018). Read more (PubMed: 29626186) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"