Anti-UCP1 antibody (ab23841)
Key features and details
- Rabbit polyclonal to UCP1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-UCP1 antibody
See all UCP1 primary antibodies -
Description
Rabbit polyclonal to UCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Hamster, Dog, Human, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human UCP1 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24282) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
Tissue specificity
Brown adipose tissue. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
- SwissProt: P04633 Rat
- Unigene: 249211 Human
see all -
Form
UCP1 is preferentially expressed in brown adipose tissue -
Alternative names
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
Images
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Western blot - Anti-UCP1 antibody (ab23841)All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml
Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
Lane 2 : Adult Rat Brown Adipose Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry - Anti-UCP1 antibody (ab23841)Image from Liu W et al., PLoS Genet. 2006;8:11958-63. Fig 5.; doi: 10.1371/journal.pgen.1003626 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (112)
ab23841 has been referenced in 112 publications.
- Giori IG et al. Enalapril and treadmill running reduce adiposity, but only the latter causes adipose tissue browning in mice. J Cell Physiol 236:900-910 (2021). PubMed: 32617979
- Lee DH et al. Withaferin A exerts an anti-obesity effect by increasing energy expenditure through thermogenic gene expression in high-fat diet-fed obese mice. Phytomedicine 82:153457 (2021). PubMed: 33444942
- Pan XX et al. Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-?. Front Cell Dev Biol 9:637424 (2021). PubMed: 33748126
- Whitehead A et al. Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis. Nat Commun 12:1905 (2021). PubMed: 33772024
- Voisin M et al. Inhibiting LXRa phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice. Commun Biol 4:420 (2021). PubMed: 33772096