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Anti-UCP1 antibody (ab23841)

Reviews (9)Q&A (10)References (118)
Western blot - Anti-UCP1 antibody (ab23841)

    Key features and details

    • Rabbit polyclonal to UCP1
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-UCP1 antibody [EPR23004-34] (ab234430)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-UCP1 antibody
      See all UCP1 primary antibodies

    • Description

      Rabbit polyclonal to UCP1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat
      Predicted to work with: Hamster, Dog, Human, Chimpanzee

    • Immunogen

      Synthetic peptide corresponding to Human UCP1 aa 100-200 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab24282)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23841 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (4)
    Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

    Target

    • Function

      UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

    • Tissue specificity

      Brown adipose tissue.

    • Sequence similarities

      Belongs to the mitochondrial carrier family.
      Contains 3 Solcar repeats.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Form

      UCP1 is preferentially expressed in brown adipose tissue

    • Alternative names

      • mitochondrial brown fat uncoupling protein antibody
      • Mitochondrial brown fat uncoupling protein 1 antibody
      • SLC25A7 antibody
      • Solute carrier family 25 member 7 antibody
      • Thermogenin antibody
      • UCP 1 antibody
      • UCP antibody
      • UCP1 antibody
      • UCP1_HUMAN antibody
      • uncoupling protein 1 (mitochondrial, proton carrier) antibody
      • Uncoupling protein 1 antibody
      see all

    Images

    • Western blot - Anti-UCP1 antibody (ab23841)
      Western blot - Anti-UCP1 antibody (ab23841)
      All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml

      Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
      Lane 2 : Adult Rat Brown Adipose Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa
      Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    References (118)

    Publishing research using ab23841? Please let us know so that we can cite the reference in this datasheet.

    ab23841 has been referenced in 118 publications.

    • Giori IG  et al. Enalapril and treadmill running reduce adiposity, but only the latter causes adipose tissue browning in mice. J Cell Physiol 236:900-910 (2021). PubMed: 32617979
    • Lee DH  et al. Withaferin A exerts an anti-obesity effect by increasing energy expenditure through thermogenic gene expression in high-fat diet-fed obese mice. Phytomedicine 82:153457 (2021). PubMed: 33444942
    • Pan XX  et al. Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-?. Front Cell Dev Biol 9:637424 (2021). PubMed: 33748126
    • Whitehead A  et al. Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis. Nat Commun 12:1905 (2021). PubMed: 33772024
    • Voisin M  et al. Inhibiting LXRa phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice. Commun Biol 4:420 (2021). PubMed: 33772096
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 19 Abreviews or Q&A

    Western blot abreview for Anti-UCP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brown fat)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brown fat
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 03 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-UCP1 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brown adipose tissue and aortic root)
    Permeabilization
    Yes - Triton X-100 (0,1% in PBS)
    Specification
    brown adipose tissue and aortic root
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 04 2017

    Western blot abreview for Anti-UCP1 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human pre-adipocytes overexpressing hUCP1)
    Gel Running Conditions
    Non-reduced Denaturing (4-15%)
    Loading amount
    30 µg
    Specification
    Human pre-adipocytes overexpressing hUCP1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Morten Lundh

    Verified customer

    Submitted Jul 18 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (A549 Lung cance cells)
    Specification
    A549 Lung cance cells
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    Submitted Feb 20 2015

    Western blot abreview for Anti-UCP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10.5%)
    Sample
    Mouse Tissue lysate - other (brown fat)
    Specification
    brown fat
    Treatment
    cold stress
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kun Dong

    Verified customer

    Submitted Jul 09 2013

    Question

    I’m very interested in purchasing anti-UCP1 antibody (ab23841) with custom fluorescence conjugate like Alexa Fluor 647. I want to know how long it will take to get fluorescence-conjugated anti-UCP1 antibody. I also want to know how to place the order like this and how much it will cost.

    Read More
    Answer

    Thank you for your inquiry.


    We do not currently offer this antibody directly conjugated to a fluorophore.I could see if the lab would be able to generate this for you, but we do not carry Alexa Fluors. We may be able to provide this service if you order in bulk (>10 units) with a Cy5 conjugation. If you would be interested in this, please email my colleage Andrea who handles bulk requests at mailto:us.sales@abcam.com.


    Alternatively, you could do the labeling yourself using one of our 3-step EasyLink conjugation kits. Again, we don't carry Alexa Fluors, but you would be able to label Cy5.


    https://www.abcam.com/index.html?pageconfig=resource&rid=13148


    In order to use the conjugation kit, like ab102879 ($335.00), with UCP1 ab23841, you would first need to remove some of the BSA in the antibody as this can interfere with the labeling reaction. See our FAQs:


    https://www.abcam.com/index.html?pageconfig=resource&rid=13156#4


    https://www.abcam.com/EasyLink-Cy5-5-Conjugation-Kit-3-x-100%C2%B5g-Cy5-5-ab102879.html


    You can remove the BSA using a purification kit like ab102783 (1 purification, $221.00) or ab102784 (3 purifications, $438.00).


    https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html


    https://www.abcam.com/Antibody-Purification-kit-3-purifications-ab102784.html


    I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    Have you done a blocking experiment with the antibody and peptide? Do you have an image?

    Read More
    Answer

    Thank you for contacting us.

    I've attached one of our blocking experiments with the antibody and peptide combination.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    Ab47431 gave a band around 50 kDa instead of 150 kDa, and ab23841 did not show any bands.

    Read More
    Answer

    Thank you very much for your call today and for letting us know about the trouble with these antibodies.

    I'm sending a free of charge vial of ab14571 on the order *** which should arrive on Tuesday, to replace the vial of ab47431. Please keep me updated about the results with ab23841 after trying the alterations we discussed.

    I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Question

    The most recent vial failed in western blotting at 1/1000 (no signal) compared to an earlier lot that worked well at 1/10,000.

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    WB with mouse brown adipocytes
    sees band at 37 kDa, but should be at 33 kDa
    other Ab from another company detects right band size
    treatment of cells should increase band intensity, but this is not seen either
    30 ug protein loaded
    Ab: 1/1000
    secondary: OK

    Read More
    Answer

    Thank you for contacting us.

    Your credit note ID is xxx.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    1-10 of 19 Abreviews or Q&A

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