Anti-UCP1 antibody (ab23841)

Reviews (9)Q&A (10)References (65)

Overview

  • Product name
    Anti-UCP1 antibody
    See all UCP1 primary antibodies
  • Description
    Rabbit polyclonal to UCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog
    Predicted to work with: Hamster, Human, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab24282.)

  • Positive control
    • WB: Mouse and rat brown adipose tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab23841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/1000.

Target

  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity
    Brown adipose tissue.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
    see all
  • Form
    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • Western blot - Anti-UCP1 antibody (ab23841)
    Western blot - Anti-UCP1 antibody (ab23841)
    All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml

    Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
    Lane 2 : Adult Rat Brown Adipose Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab23841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (ab23841)Image from Liu W et al., PLoS Genet. 2006;8:11958-63. Fig 5.; doi: 10.1371/journal.pgen.1003626

    ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.

     

References

This product has been referenced in:
  • Dimitriadis GK  et al. Effects of visfatin on brown adipose tissue energy regulation using T37i cells. Cytokine 113:248-255 (2019). Read more (PubMed: 30060995) »
  • Sepa-Kishi DM  et al. Cold acclimation enhances UCP1 content, lipolysis, and triacylglycerol resynthesis, but not mitochondrial uncoupling and fat oxidation, in rat white adipocytes. Am J Physiol Cell Physiol 316:C365-C376 (2019). Read more (PubMed: 30624981) »
See all 65 Publications for this product

Publishing research using ab23841? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 19 Abreviews or Q&A

Question

I’m very interested in purchasing anti-UCP1 antibody (ab23841) with custom fluorescence conjugate like Alexa Fluor 647. I want to know how long it will take to get fluorescence-conjugated anti-UCP1 antibody. I also want to know how to place the order like this and how much it will cost.

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Answer

Thank you for your inquiry.


We do not currently offer this antibody directly conjugated to a fluorophore.I could see if the lab would be able to generate this for you, but we do not carry Alexa Fluors. We may be able to provide this service if you order in bulk (>10 units) with a Cy5 conjugation. If you would be interested in this, please email my colleage Andrea who handles bulk requests at mailto:us.sales@abcam.com.


Alternatively, you could do the labeling yourself using one of our 3-step EasyLink conjugation kits. Again, we don't carry Alexa Fluors, but you would be able to label Cy5.


https://www.abcam.com/index.html?pageconfig=resource&rid=13148


In order to use the conjugation kit, like ab102879 ($335.00), with UCP1 ab23841, you would first need to remove some of the BSA in the antibody as this can interfere with the labeling reaction. See our FAQs:


https://www.abcam.com/index.html?pageconfig=resource&rid=13156#4


https://www.abcam.com/EasyLink-Cy5-5-Conjugation-Kit-3-x-100%C2%B5g-Cy5-5-ab102879.html


You can remove the BSA using a purification kit like ab102783 (1 purification, $221.00) or ab102784 (3 purifications, $438.00).


https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html


https://www.abcam.com/Antibody-Purification-kit-3-purifications-ab102784.html


I hope this information helps. Please contact us with any other questions.

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Western blot abreview for Anti-UCP1 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brown fat)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brown fat
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Dec 03 2018

Immunohistochemistry (Frozen sections) abreview for Anti-UCP1 antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brown adipose tissue and aortic root)
Permeabilization
Yes - Triton X-100 (0,1% in PBS)
Specification
brown adipose tissue and aortic root
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 04 2017

Western blot abreview for Anti-UCP1 antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human pre-adipocytes overexpressing hUCP1)
Gel Running Conditions
Non-reduced Denaturing (4-15%)
Loading amount
30 µg
Specification
Human pre-adipocytes overexpressing hUCP1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Morten Lundh

Verified customer

Submitted Jul 18 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-UCP1 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (A549 Lung cance cells)
Specification
A549 Lung cance cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Read More

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 20 2015

Western blot abreview for Anti-UCP1 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10.5%)
Sample
Mouse Tissue lysate - other (brown fat)
Specification
brown fat
Treatment
cold stress
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Dr. Kun Dong

Verified customer

Submitted Jul 09 2013

Question

Have you done a blocking experiment with the antibody and peptide? Do you have an image?

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Answer

Thank you for contacting us.

I've attached one of our blocking experiments with the antibody and peptide combination.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Ab47431 gave a band around 50 kDa instead of 150 kDa, and ab23841 did not show any bands.

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Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

I'm sending a free of charge vial of ab14571 on the order *** which should arrive on Tuesday, to replace the vial of ab47431. Please keep me updated about the results with ab23841 after trying the alterations we discussed.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

The most recent vial failed in western blotting at 1/1000 (no signal) compared to an earlier lot that worked well at 1/10,000.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

WB with mouse brown adipocytes
sees band at 37 kDa, but should be at 33 kDa
other Ab from another company detects right band size
treatment of cells should increase band intensity, but this is not seen either
30 ug protein loaded
Ab: 1/1000
secondary: OK

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Answer

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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