Recombinant
RabMAb

Recombinant Anti-UCP1 antibody [EPR20381] - BSA and Azide free (ab222397)

Overview

  • Product name

    Anti-UCP1 antibody [EPR20381] - BSA and Azide free
    See all UCP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20381] to UCP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse UCP1 aa 100-200. The exact sequence is proprietary.
    Database link: P12242

  • Positive control

    • WB: Mouse and rat brown adipose tissue lysates. IHC-P: Mouse and rat brown adipose tissue. IP: Rat brown adipose tissue lysate.
  • General notes

    Ab222397 is the carrier-free version of ab209483. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222397 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab222397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity

    Brown adipose tissue.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Form

    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names

    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on mouse brown adipose tissue is observed [PMID: 24753268] [PMID: 23824424].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209483).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on mouse brown adipose tissue, no staining on adjacent white adipose tissue [PMID: 24753268] [PMID: 23824424].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209483).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on rat brown adipose tissue is observed [PMID: 24753268] [PMID: 23824424].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209483).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on rat brown adipose tissue, no staining on adjacent white adipose tissue [PMID: 24753268] [PMID: 23824424].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209483).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • UCP1 was immunoprecipitated from 0.35 mg of rat brown adipose lysate with ab209483 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209483 at 1/5000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat brown adipose lysate, 10 ug (Input).

    Lane 2: ab209483 IP in rat brown adipose lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209483 in rat brown adipose lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209483).

References

ab222397 has not yet been referenced specifically in any publications.

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