Overview

  • Product name

  • Description

    Rabbit polyclonal to UCP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human UCP2 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107707)

  • Positive control

    • This antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab97931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity

    Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BMIQ4 antibody
    • Mitochondrial uncoupling protein 2 antibody
    • SLC25A8 antibody
    • Solute carrier family 25 member 8 antibody
    • UCP 2 antibody
    • ucp2 antibody
    • UCP2_HUMAN antibody
    • UCPH antibody
    • Uncoupling protein 2 antibody
    • Uncoupling protein 2 mitochondrial proton carrier antibody
    see all

Images

  • All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Wei W  et al. Rosuvastatin Alleviates Ischemia/Reperfusion Injury in Cardiomyocytes by Downregulating Hsa-miR-24-3p to Target Upregulated Uncoupling Protein 2. Cell Reprogram 21:99-107 (2019). Read more (PubMed: 30835496) »
  • Zhong X  et al. UCP2 alleviates tubular epithelial cell apoptosis in lipopolysaccharide-induced acute kidney injury by decreasing ROS production. Biomed Pharmacother 115:108914 (2019). Read more (PubMed: 31071510) »
See all 12 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4, 5 min at RT
Specification
DU145 prostate cancer cell line
Blocking step
(agent) for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Dimitra Kalamida

Verified customer

Submitted Nov 20 2017

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Human Cell lysate - other (Heterologously expressed in Lactococcus lactis)
Specification
Heterologously expressed in Lactococcus lactis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Martin King

Verified customer

Submitted Jul 31 2014

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2014

Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab97931 has never been tested in EM. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

I can also confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are none to provide on this occasion for ab97931.

I can also provide you with a 5% discount if you wish to purchase this antibody to try in EM. In order to obtain this discount, please use the following code when you place the order:

########


Also, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Answer

Thank you for contacting us.

We currently offer one product which has been tested in IHC-P and is predicted to work in primates. Ab97931 (https://www.abcam.com/UCP2-antibody-ab97931.html).

Though predicted to work based upon sequence homology, this product has not been tested in primate.For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


I hope this information is helpful to you. If you are interested in this offer please let me know. Also, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Human endotherial cells)
Loading amount
15 µg
Specification
Human endotherial cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 16 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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