- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-UCP3 antibody
See all UCP3 primary antibodies -
DescriptionRabbit polyclonal to UCP3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human UCP3 aa 254-267 conjugated to keyhole limpet haemocyanin.
Database link: P55916 -
General notesStorage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 15mM sodium azide.
Constituents: 0.01M PBS, 1% BSA. pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody is affinity-purified using the immunogen peptide immobilized on agarose.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab10985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 34 kDa. Predicted molecular weight migrates at ~50 kDa in positive control lysate. (Protein is glycosylated) | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | 1/3000. |
Target
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FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
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Tissue specificityOnly in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
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Involvement in diseaseDefects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
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Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 7352 Human
- Entrez Gene: 22229 Mouse
- Entrez Gene: 25708 Rat
- Omim: 602044 Human
- SwissProt: P55916 Human
- SwissProt: P56501 Mouse
- SwissProt: P56499 Rat
- Unigene: 101337 Human
see all -
FormUCP3 is preferentially expressed in muscle
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Alternative names
- Mitochondrial uncoupling protein 3 antibody
- SLC25A9 antibody
- Solute carrier family 25 member 9 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (ab10985)ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References
This product has been referenced in:
- Trewin AJ et al. Acute exercise alters skeletal muscle mitochondrial respiration and H2O2 emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men. PLoS One 12:e0188421 (2017). Read more (PubMed: 29161316) »
- Cai C et al. Loss-of-function myostatin mutation increases insulin sensitivity and browning of white fat in Meishan pigs. Oncotarget 8:34911-34922 (2017). Read more (PubMed: 28432282) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"