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Our Abpromise guarantee covers the use of ab3477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Mouse UCP3 peptide (ab4994).
By Western blot, this antibody detects an ~30 kDa protein representing UCP 3 from human and mouse skeletal muscle lysate.
The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection.
Western blot detection of recombinant human UCP-3 using ab3477.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"