Anti-UCP5 antibody (ab221123)

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Immunocytochemistry/ Immunofluorescence - Anti-UCP5 antibody (ab221123)

    Key features and details

    • Rabbit polyclonal to UCP5
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-UCP5 antibody
      See all UCP5 primary antibodies

    • Description

      Rabbit polyclonal to UCP5

    • Host species

      Rabbit

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse

    • Immunogen

      Recombinant fragment corresponding to Human UCP5 aa 19-43.
      Sequence:

      AAVIVSGHQKSTTVSHEMSGLNWKP


      Database link: O95258
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • ICC/IF: U-251 MG cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    Applications

    Our Abpromise guarantee covers the use of ab221123 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Relevance

      Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. The protein can be found in many tissues with the greatest abundance in brain and testis and has an N-terminal hydrophobic domain that is not present in other UCPs.

    • Cellular localization

      Mitochondrial

    • Database links

      • Alternative names

        • BMCP1 antibody
        • Brain mitochondrial carrier protein 1 antibody
        • Mitochondrial Uncoupling Protein 5 antibody
        • Solute Carrier Family 25 Member 14 antibody

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-UCP5 antibody (ab221123)
        Immunocytochemistry/ Immunofluorescence - Anti-UCP5 antibody (ab221123)

        Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized U-251 MG cells labeling UCP5 with ab221123 at 4 μg/mL (green).

      References (0)

      Publishing research using ab221123? Please let us know so that we can cite the reference in this datasheet.

      ab221123 has not yet been referenced specifically in any publications.

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