Overview

  • Product name

  • Description

    Rabbit polyclonal to UCP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human UCP5 aa 55-111.
    Database link: O95258

  • Positive control

    • ICC/IF: MCF7 cells. IHC-P: Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab238856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Relevance

    Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. The protein can be found in many tissues with the greatest abundance in brain and testis and has an N-terminal hydrophobic domain that is not present in other UCPs.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • BMCP1 antibody
    • Brain mitochondrial carrier protein 1 antibody
    • Mitochondrial Uncoupling Protein 5 antibody
    • Solute Carrier Family 25 Member 14 antibody

Images

  • MCF7 (Human breast adenocarcinoma cell line) cells stained for UCP5 (Green) using ab238856 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

  • Paraffin-embedded human testis tissue stained for UCP5 using ab238856 at 1/100 dilution in immunohistochemical analysis.

References

ab238856 has not yet been referenced specifically in any publications.

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