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Prepared 20 mM stock in DMSO, then further diluted to 100 uM in ACSF (artificial cerebrospinal fluid, recipe below). In this solution, the UCPH-101 crashed out. Tried sonicating but it would not go back into solution. Is there any way to dissolve the UCPH-101 into ACSF?
ACSF- pH 7.4
KCl: 2.5 mM
MgSO4: 1 mM
NaHCO3: 26 mM
NaPO4: 4 mM
glucose: 20 mM
ascorbic acid: 0.4 mM
CaCl: 2 mM
Asked on Mar 30 2012
Thank you for your calls earlier this week and for your patience while I have been in touch with the Abcam Biochemicals lab regarding your enquiry.
I have heard from my colleagues:
Unfortunately this compound has not be tried in ACSF. However, we do know that UCPH-101 can be diluted to 100 uM (using heatingin water bath and sonicating) in Hank's buffered Saline Solution supplemented with 20 mM HEPES (pH 7.4) and also in Krebs buffer ( [140 mM NaCl/4.7 mM KCl/2.5 mM CaCl2/1.2 mM MgCl2/11 mM HEPES/10 mM D-Glucose, pH 7.4].). It may be helpful to try gently heating the solution.
After a literature search, we could not find any further information about this compound's solubility in ACSF.
I am sorry that I don't have any more information, but if there is anything else that we can do for you please let me know, and I'll be happy to help.
Answered on Mar 30 2012