Key features and details
- Rabbit polyclonal to UEV1A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-UEV1A antibody
DescriptionRabbit polyclonal to UEV1A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to 15 C terminal amino acids of Human UEV1 (170 aa isoform a)(GenBank accession no. NP_954595).
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesPurified by ion exchange chromatography.
Our Abpromise guarantee covers the use of ab25962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).|
RelevanceUbiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
- CIR1 antibody
- CROC1 antibody
- CROC1A antibody
Lane 1 : Anti-UEV1A antibody (ab25962) at 1 µg/ml
Lane 2 : Anti-UEV1A antibody (ab25962) at 2 µg/ml
Lane 3 : Anti-UEV1A antibody (ab25962) at 4 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 19 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
ab25962 has not yet been referenced specifically in any publications.