Recombinant
RabMAb

Anti-UFC1 antibody [EPR15014-102] (ab189252)

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Overview

  • Product name
    Anti-UFC1 antibody [EPR15014-102]
    See all UFC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15014-102] to UFC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UFC1 aa 1-150. The exact sequence is proprietary.
    Database link: Q9Y3C8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human fetal liver, U87-MG, MCF7, A549, RAW 264.7, NIH 3T3 cell lysates; Human colon and Human transitional cell carcinoma of bladder tissues; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15014-102
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Western blot - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/5000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (ab189252)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Immunocytochemistry/ Immunofluorescence - Anti-UFC1 antibody [EPR15014-102] (ab189252)

    Immunofluorescent analysis of MCF7 cells(4% paraformaldehyde-fixed)  labeling UFC1 with ab189252 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunoprecipitation - Anti-UFC1 antibody [EPR15014-102] (ab189252)
    Immunoprecipitation - Anti-UFC1 antibody [EPR15014-102] (ab189252)

    Western blot analysis of immunoprecipitation pellet from MCF7cell lysate immunoprecipitated using ab189252 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab189252 has not yet been referenced specifically in any publications.

Publishing research using ab189252? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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