Overview

  • Product name

    Anti-UFC1 antibody [EPR15014]
    See all UFC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15014] to UFC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UFC1 aa 1-150. The exact sequence is proprietary.
    Database link: Q9Y3C8

  • Positive control

    • Human fetal liver, A549, U-87 MG, MCF7, C6, PC12, Raw 264.7 and NIH 3T3 lysates; Human colon and hepatocellular carcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15014
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1000 µg

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Nahorski MS  et al. Biallelic UFM1 and UFC1 mutations expand the essential role of ufmylation in brain development. Brain 141:1934-1945 (2018). Read more (PubMed: 29868776) »
  • Colin E  et al. Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy. Am J Hum Genet 99:695-703 (2016). Read more (PubMed: 27545681) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189251.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up