Anti-UFD1L antibody (ab155003)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-UFD1L antibody
See all UFD1L primary antibodies -
DescriptionRabbit polyclonal to UFD1L
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 45 and 307 of Human UFD1L (Uniprot ID: Q92890).
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Positive control
- A431 whole cell lysate; A431 cells; H1299 xenograft
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General notesKeep as a concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 35 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
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Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
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PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
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Sequence similaritiesBelongs to the UFD1 family.
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Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
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Database links
- Entrez Gene: 7353 Human
- Omim: 601754 Human
- SwissProt: Q92890 Human
- Unigene: 474213 Human
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Alternative names
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-UFD1L antibody (ab155003)Immunofluorescent analysis of methanol-fixed A431 cells, labeling UFD1L with ab155003 at 1/200 dilution. Lower image shows costaining with Hoechst 33342. -
Western blot - Anti-UFD1L antibody (ab155003)Anti-UFD1L antibody (ab155003) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFD1L antibody (ab155003)Immunohistochemical analysis of paraffin-embedded H1299 xenograft labeling UFD1L protein with ab155003 at 1/500.
Protocols
References
ab155003 has not yet been referenced specifically in any publications.
