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Rabbit polyclonal to UFD1L
Recombinant fragment corresponding to a region within amino acids 45 and 307 of Human UFD1L (Uniprot ID: Q92890).
A431 whole cell lysate; A431 cells; H1299 xenograft
Keep as a concentrated solution.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
1/100 - 1/1000.
1/100 - 1/1000.
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Protein degradation; proteasomal ubiquitin-dependent pathway.
Belongs to the UFD1 family.
Nucleus. Cytoplasm > cytosol.
Information by UniProt
UB fusion protein 1 antibody
Ubiquitin fusion degradation 1 like (yeast) antibody
Ubiquitin fusion degradation 1 like antibody
Immunocytochemistry/ Immunofluorescence - Anti-UFD1L antibody (ab155003)
Immunofluorescent analysis of methanol-fixed A431 cells, labeling UFD1L with ab155003 at 1/200 dilution. Lower image shows costaining with Hoechst 33342.
Western blot - Anti-UFD1L antibody (ab155003)
Anti-UFD1L antibody (ab155003) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 35 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFD1L antibody (ab155003)
Immunohistochemical analysis of paraffin-embedded H1299 xenograft labeling UFD1L protein with ab155003 at 1/500.
has not yet been referenced specifically in any publications.
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