Overview

  • Product name
  • Description
    Rabbit polyclonal to UFD1L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 45 and 307 of Human UFD1L (Uniprot ID: Q92890).

  • Positive control
    • A431 whole cell lysate; A431 cells; H1299 xenograft
  • General notes
    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
  • Tissue specificity
    Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
  • Pathway
    Protein degradation; proteasomal ubiquitin-dependent pathway.
  • Sequence similarities
    Belongs to the UFD1 family.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • UB fusion protein 1 antibody
    • Ubiquitin fusion degradation 1 like (yeast) antibody
    • Ubiquitin fusion degradation 1 like antibody
    • Ubiquitin fusion degradation protein 1 homolog antibody
    • UFD1 antibody
    • UFD1_HUMAN antibody
    • UFD1L antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed A431 cells, labeling UFD1L with ab155003 at 1/200 dilution. Lower image shows costaining with Hoechst 33342.
  • Anti-UFD1L antibody (ab155003) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded H1299 xenograft labeling UFD1L protein with ab155003 at 1/500.

References

ab155003 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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