Key features and details
- Rabbit polyclonal to UFD1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UFD1L antibody
See all UFD1L primary antibodies
DescriptionRabbit polyclonal to UFD1L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 45 and 307 of Human UFD1L (Uniprot ID: Q92890).
- A431 whole cell lysate; A431 cells; H1299 xenograft
General notesKeep as a concentrated solution.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
Sequence similaritiesBelongs to the UFD1 family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
Anti-UFD1L antibody (ab155003) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed A431 cells, labeling UFD1L with ab155003 at 1/200 dilution. Lower image shows costaining with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft labeling UFD1L protein with ab155003 at 1/500.
ab155003 has been referenced in 1 publication.
- Skrott Z et al. Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature 552:194-199 (2017). PubMed: 29211715