Product nameAnti-UFD1L antibody
See all UFD1L primary antibodies
DescriptionRabbit polyclonal to UFD1L
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human UFD1L aa 175-225. The exact sequence is proprietary. NP_005650.2
Database link: Q92890
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264243 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 35 kDa.|
FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
Sequence similaritiesBelongs to the UFD1 family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
All lanes : Anti-UFD1L antibody (ab264243) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 3 seconds
UFD1L was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264243 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264243 at 0.4 μg/ml.
Lane 1: ab264243 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264243 has not yet been referenced specifically in any publications.