Key features and details
- Rabbit polyclonal to UFD1L
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UFD1L antibody
See all UFD1L primary antibodies
DescriptionRabbit polyclonal to UFD1L
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human UFD1L aa 257-307. The exact sequence is proprietary. NP_005650.2
Database link: Q92890
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 35 kDa.|
FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
Sequence similaritiesBelongs to the UFD1 family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
All lanes : Anti-UFD1L antibody (ab264244) at 0.04 µg/ml
Lane 1 : HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 35 kDa
Exposure time: 3 seconds
UFD1L was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264244 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264244 at 0.4 μg/ml.
Lane 1: ab264244 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264244 has not yet been referenced specifically in any publications.