Key features and details
- Rabbit polyclonal to UFD1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UFD1L antibody
See all UFD1L primary antibodies
DescriptionRabbit polyclonal to UFD1L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell IHC-P: H441 xenograft
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
Sequence similaritiesBelongs to the UFD1 family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE
ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe
ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.
ab96648 has been referenced in 1 publication.
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924