Overview

  • Product name

    Anti-UFD1L antibody [EPR12847] - N-terminal
    See all UFD1L primary antibodies
  • Description

    Rabbit monoclonal [EPR12847] to UFD1L - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UFD1L aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q92890

  • Positive control

    • Raji and HeLa cell lysates; A431 and 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35, 40 kDa (predicted molecular weight: 35 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
  • Tissue specificity

    Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
  • Pathway

    Protein degradation; proteasomal ubiquitin-dependent pathway.
  • Sequence similarities

    Belongs to the UFD1 family.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • UB fusion protein 1 antibody
    • Ubiquitin fusion degradation 1 like (yeast) antibody
    • Ubiquitin fusion degradation 1 like antibody
    • Ubiquitin fusion degradation protein 1 homolog antibody
    • UFD1 antibody
    • UFD1_HUMAN antibody
    • UFD1L antibody
    see all

Images

  • All lanes : Anti-UFD1L antibody [EPR12847] - N-terminal (ab181080) at 1/2000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 1/1000 dilution per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling UFD1L with ab181080 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Conterstained with Dapi (blue).

  • Flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling UFD1L with ab181080 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit IgG

References

This product has been referenced in:

  • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). Read more (PubMed: 28985237) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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