Anti-UFL1 antibody (ab123500)
Key features and details
- Rabbit polyclonal to UFL1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UFL1 antibody
See all UFL1 primary antibodies -
Description
Rabbit polyclonal to UFL1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human UFL1 aa 175-225.
Database link: NP_056138.1 -
Positive control
- HeLa whole cell lysate
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General notes
This product was previously labelled as K0776
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3. -
Tissue specificity
Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC. -
Sequence similarities
Belongs to the UFL1 family. -
Post-translational
modificationsUbiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome. -
Cellular localization
Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3. - Information by UniProt
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Database links
- Entrez Gene: 462889 Chimpanzee
- Entrez Gene: 101150106 Gorilla
- Entrez Gene: 100065945 Horse
- Entrez Gene: 23376 Human
- Entrez Gene: 67490 Mouse
- Entrez Gene: 313115 Rat
- Entrez Gene: 704454 Rhesus monkey
- Omim: 613372 Human
see all -
Alternative names
- E3 UFM1-protein ligase 1 antibody
- KIAA0776 antibody
- LZAP-binding protein antibody
see all
Images
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Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.
Subsequent Western blot detection used an antibody which recognized a downstream epitope of UFL1 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab123500 has not yet been referenced specifically in any publications.