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    ufl1-antibody-ab123500.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins
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Anti-UFL1 antibody (ab123500)

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Immunoprecipitation - Anti-UFL1 antibody (ab123500)

    Key features and details

    • Rabbit polyclonal to UFL1
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human UFL1 protein (ab161615)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-UFL1 antibody
      See all UFL1 primary antibodies
    • Description

      Rabbit polyclonal to UFL1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Human UFL1 aa 175-225.
      Database link: NP_056138.1

    • Positive control

      • HeLa whole cell lysate
    • General notes

       This product was previously labelled as K0776

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.00
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ub-like Proteins
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • Other E3 Ligases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab29545)
    • Recombinant Protein

      • Recombinant Human UFL1 protein (ab161615)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab123500 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at 2-10 µg/mg of lysate.
    Notes
    IP
    Use at 2-10 µg/mg of lysate.

    Target

    • Function

      E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3.
    • Tissue specificity

      Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC.
    • Sequence similarities

      Belongs to the UFL1 family.
    • Post-translational
      modifications

      Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.
    • Cellular localization

      Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3.
    • Target information above from: UniProt accession O94874 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 462889 Chimpanzee
      • Entrez Gene: 101150106 Gorilla
      • Entrez Gene: 100065945 Horse
      • Entrez Gene: 23376 Human
      • Entrez Gene: 67490 Mouse
      • Entrez Gene: 313115 Rat
      • Entrez Gene: 704454 Rhesus monkey
      • Omim: 613372 Human
      • SwissProt: O94874 Human
      • SwissProt: Q8CCJ3 Mouse
      • SwissProt: B2GV24 Rat
      • Unigene: 149367 Human
      • Unigene: 226295 Mouse
      • Unigene: 12784 Rat
      see all
    • Alternative names

      • E3 UFM1-protein ligase 1 antibody
      • KIAA0776 antibody
      • LZAP-binding protein antibody
      • Maxer antibody
      • NLBP antibody
      • RCAD antibody
      • UFL1 antibody
      • UFL1_HUMAN antibody
      • UFM1 specific ligase 1 antibody
      see all

    Images

    • Immunoprecipitation - Anti-UFL1 antibody (ab123500)
      Immunoprecipitation - Anti-UFL1 antibody (ab123500)

      Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.
      Subsequent Western blot detection used an antibody which recognized a downstream epitope of UFL1 at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Protocols

    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab123500? Please let us know so that we can cite the reference in this datasheet.

    ab123500 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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