Anti-UFL1 antibody (ab123500)
Key features and details
- Rabbit polyclonal to UFL1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-UFL1 antibody
See all UFL1 primary antibodies -
Description
Rabbit polyclonal to UFL1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human UFL1 aa 175-225.
Database link: NP_056138.1 -
Positive control
- HeLa whole cell lysate
-
General notes
This product was previously labelled as K0776
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3. -
Tissue specificity
Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC. -
Sequence similarities
Belongs to the UFL1 family. -
Post-translational
modificationsUbiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome. -
Cellular localization
Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3. - Information by UniProt
-
Database links
- Entrez Gene: 462889 Chimpanzee
- Entrez Gene: 101150106 Gorilla
- Entrez Gene: 100065945 Horse
- Entrez Gene: 23376 Human
- Entrez Gene: 67490 Mouse
- Entrez Gene: 313115 Rat
- Entrez Gene: 704454 Rhesus monkey
- Omim: 613372 Human
see all -
Alternative names
- E3 UFM1-protein ligase 1 antibody
- KIAA0776 antibody
- LZAP-binding protein antibody
see all
Images
-
Immunoprecipitation - Anti-UFL1 antibody (ab123500)
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.
Subsequent Western blot detection used an antibody which recognized a downstream epitope of UFL1 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab123500 has not yet been referenced specifically in any publications.