Overview

  • Product name

  • Description

    Rabbit polyclonal to UFL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human UFL1 aa 175-225.
    Database link: NP_056138.1

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as K0776

     

Properties

Applications

Our Abpromise guarantee covers the use of ab123500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3.
    • Tissue specificity

      Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC.
    • Sequence similarities

      Belongs to the UFL1 family.
    • Post-translational
      modifications

      Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.
    • Cellular localization

      Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 UFM1-protein ligase 1 antibody
      • KIAA0776 antibody
      • LZAP-binding protein antibody
      • Maxer antibody
      • NLBP antibody
      • RCAD antibody
      • UFL1 antibody
      • UFL1_HUMAN antibody
      • UFM1 specific ligase 1 antibody
      see all

    Images

    • Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.
      Subsequent Western blot detection used an antibody which recognized a downstream epitope of UFL1 at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab123500 has not yet been referenced specifically in any publications.

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