Overview

  • Product name

    Anti-UFL1 antibody - C-terminal
    See all UFL1 primary antibodies
  • Description

    Rabbit polyclonal to UFL1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human UFL1 aa 744-794 (C terminal). The exact sequence is proprietary. (NP_056138.1).
    Database link: O94874-1

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate (ab150035).
  • General notes

     This product was previously labelled as K0776

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-UFL1 antibody - C-terminal (ab226216) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 90 kDa


    Exposure time: 30 seconds
  • UFL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226216 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226216 at 0.4 µg/ml.

    Lane 1: ab226216 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds

References

ab226216 has not yet been referenced specifically in any publications.

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