Overview

  • Product name

    Anti-UFL1 antibody - N-terminal
    See all UFL1 primary antibodies
  • Description

    Rabbit polyclonal to UFL1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human UFL1 (N terminal). The exact sequence is proprietary.
    Database link: O94874

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2, Jurkat, Raji and NCI-H929 whole cell extracts.
  • General notes

     This product was previously labelled as K0776

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 90 kDa.

Target

  • Function

    E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3.
  • Tissue specificity

    Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC.
  • Sequence similarities

    Belongs to the UFL1 family.
  • Post-translational
    modifications

    Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.
  • Cellular localization

    Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 UFM1-protein ligase 1 antibody
    • KIAA0776 antibody
    • LZAP-binding protein antibody
    • Maxer antibody
    • NLBP antibody
    • RCAD antibody
    • UFL1 antibody
    • UFL1_HUMAN antibody
    • UFM1 specific ligase 1 antibody
    see all

Images

  • All lanes : Anti-UFL1 antibody - N-terminal (ab227506) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 90 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-UFL1 antibody - N-terminal (ab227506) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 90 kDa



    7.5% SDS-PAGE gel.

References

ab227506 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227506.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up