Key features and details
- Rabbit polyclonal to UFSP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UFSP2 antibody
See all UFSP2 primary antibodies
DescriptionRabbit polyclonal to UFSP2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human UFSP2 aa 63-343.
WPSSDINTIPGELTDASACKNILRFIQFEPEEDIKRKFMRKKDKKLSDMH QIVNIDLMLEMSTSLAAVTPIIERESGGHHYVNMTLPVDAVISVAPEETW GKVRKLLVDAIHNQLTDMEKCILKYMKGTSIVVPEPLHFLLPGKKNLVTI SYPSGIPDGQLQAYRKELHDLFNLPHDRPYFKRSNAYHFPDEPYKDGYIR NPHTYLNPPNMETGMIYVVQGIYGYHHYMQDRIDDNGWGCAYRSLQTICS WFKHQGYTERSIPTHREIQQALVDAGDKPAT
Database link: Q9NUQ7
- WB: HepG2 and NIH/3T3 whole cell lysates; Mouse kidney lysate. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionThiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation.
Involvement in diseaseBeukes familial hip dysplasia
Sequence similaritiesBelongs to the peptidase C78 family.
Cellular localizationCytoplasm. Endoplasmic reticulum. Nucleus.
- Information by UniProt
- C4orf20 antibody
- Chromosome 4 open reading frame 20 antibody
- FLJ11200 antibody
All lanes : Anti-UFSP2 antibody (ab236790) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 53 kDa
Paraffin-embedded human kidney tissue stained for UFSP2 using ab236780 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for UFSP2 (green) using ab236790 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236790 has not yet been referenced specifically in any publications.