Anti-UGDH antibody (ab155005)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-UGDH antibody
See all UGDH primary antibodies -
Description
Rabbit polyclonal to UGDH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human UGDH aa 9-283. The exact sequence is proprietary.
Database link: O60701 -
Positive control
- WB: HepG2 whole cell lysate (ab7900) and mouse and rat liver tissue lysate. IHC-P: Human hepatoma tissue.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 55 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. -
Tissue specificity
Widely expressed. -
Pathway
Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. -
Sequence similarities
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. - Information by UniProt
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Database links
- Entrez Gene: 7358 Human
- Omim: 603370 Human
- SwissProt: O60701 Human
- Unigene: 572518 Human
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Alternative names
- GDH antibody
- UDP Glc dehydrogenase antibody
- UDP GlcDH antibody
see all
Images
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Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/10000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/2000 dilution + rat liver lysate/extract at 50 µg
Predicted band size: 55 kDa
7.5 % SDS-PAGE -
Western blot - Anti-UGDH antibody (ab155005)Anti-UGDH antibody (ab155005) at 1/10000 dilution + HepG2 whole cell lysate/extract at 30 µg
Predicted band size: 55 kDa
7.5 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab155005)Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labelling UGDH with ab155005 at a 1/500 dilution.
References
This product has been referenced in:
- Kageyama S et al. Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29339380) »
- Saito T et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat Commun 7:12030 (2016). WB . Read more (PubMed: 27345495) »
