Product nameAnti-UGDH antibody
See all UGDH primary antibodies
DescriptionRabbit polyclonal to UGDH
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Giant Panda
Synthetic peptide within Human UGDH aa 25-75. The exact sequence is proprietary. (NP_003350.1).
Database link: O60701
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226172 was affinity purified using an epitope specific to UGDH immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 55 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
Tissue specificityWidely expressed.
PathwayNucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.
Sequence similaritiesBelongs to the UDP-glucose/GDP-mannose dehydrogenase family.
- Information by UniProt
- GDH antibody
- UDP Glc dehydrogenase antibody
- UDP GlcDH antibody
All lanes : Anti-UGDH antibody (ab226172) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
UGDH was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226172 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226172 at 0.4 µg/ml.
Lane 1: ab226172 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226172 has not yet been referenced specifically in any publications.