Anti-UGDH antibody (ab226172)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-UGDH antibody
See all UGDH primary antibodies -
Description
Rabbit polyclonal to UGDH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Giant Panda
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Immunogen
Synthetic peptide within Human UGDH aa 25-75. The exact sequence is proprietary. (NP_003350.1).
Database link: O60701 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226172 was affinity purified using an epitope specific to UGDH immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 55 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. -
Tissue specificity
Widely expressed. -
Pathway
Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. -
Sequence similarities
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. - Information by UniProt
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Database links
- Entrez Gene: 422792 Chicken
- Entrez Gene: 471171 Chimpanzee
- Entrez Gene: 281564 Cow
- Entrez Gene: 479107 Dog
- Entrez Gene: 100055067 Horse
- Entrez Gene: 7358 Human
- Entrez Gene: 22235 Mouse
- Entrez Gene: 100174620 Orangutan
see all -
Alternative names
- GDH antibody
- UDP Glc dehydrogenase antibody
- UDP GlcDH antibody
see all
Images
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Western blot - Anti-UGDH antibody (ab226172)All lanes : Anti-UGDH antibody (ab226172) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-UGDH antibody (ab226172)UGDH was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226172 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226172 at 0.4 µg/ml.
Lane 1: ab226172 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
ab226172 has not yet been referenced specifically in any publications.
